A Novel Gene Transformation Technique Using Water-in-Oil Droplet in an Electrostatic Field
A Novel Gene Transformation Technique Using Water-in-Oil Droplet in an Electrostatic Field
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DOI：
10.1109/tia.2012.2229452
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发表时间：
2013-01-01
影响因子：
4.4
通讯作者：
Mizuno, Akira
中科院分区：
文献类型：
--
作者：
Asada, Atsushi;Aoki, Hironori;Mizuno, Akira
基于液滴的微全分析系统已经被许多研究人员研究并应用于生物工程。在这些系统中，单个油包水(W/O)液滴充当微反应器。要操纵W/O液滴，静电场是最有用的工具之一。例如，通过在浸泡在油中的电极之间施加直流(DC)电场，电极间距中的W/O液滴由于介电泳力和库仑力而往复运动。本文采用液滴回收法对大肠杆菌细胞进行了基因转化。在电极之间放置含有大肠杆菌和质粒脱氧核糖核酸的液滴，并施加直流电压。结果，获得了转化子。因此，证实了通过静电液滴操纵进行的基因转化。该方法可以比常规方法的样品量小。因此，我们的新型基因转染法有望成为微型化生物工程的新工具。
Droplet-based micro total analysis systems have been investigated by many researchers and used in bioengineering. In these systems, individual water-in-oil (W/O) droplets perform as microreactors. To manipulate W/O droplets, an electrostatic field is one of the most useful tools. For example, by applying a direct current (dc) electric field between electrodes soaked in oil, W/O droplets in the electrode spacings reciprocate due to dielectrophoretic force and Coulomb force. In this paper, gene transformation to Escherichia coli cells by using the droplet recycling is carried out. A droplet containing E. coli and plasmid deoxyribonucleic acid was located between the electrodes, and dc voltage was applied. As a result, the transformants were obtained. Therefore, the gene transformation by the electrostatic droplet manipulation was confirmed. In this method, sample volume can be smaller than that of conventional method. Therefore, our novel gene transfection method is expected to be a new tool in miniaturized bioengineering.