Expression of MAGE-D4, a novel MAGE family antigen, is correlated with tumor-cell proliferation of non-small cell lung cancer
Expression of MAGE-D4, a novel MAGE family antigen, is correlated with tumor-cell proliferation of non-small cell lung cancer
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DOI：
10.1016/j.lungcan.2005.08.012
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发表时间：
2006-01-01
期刊：
影响因子：
5.3
通讯作者：
Tanaka, F
中科院分区：
文献类型：
--
作者：
Ito, S;Kawano, Y;Tanaka, F
目的：MAGE-D4，最初称为 MAGE-E1，是一种新型 MAGE 家族基因，与正常组织相比，在恶性肿瘤中高水平表达。本研究旨在评估非小细胞肺癌 (NSCLC) 中 MAGE-D4 表达的临床意义。实验设计：通过免疫组织化学评估 MAGE-D4 蛋白的表达，并使用定量逆转录 PCR (RT-PCR) 研究 MAGE-D4 mRNA 表达。结果：我们评估了 NSCLC 组织中的 MAGE-D4 表达，发现与正常肺组织相比，肿瘤组织中 MAGE-D4 表达上调（平均 MAGE-D4/GAPDH 值，肿瘤组织为 0.035，正常肺组织为 0.009；p=0.002）。但不同病理阶段MAGE-D4表达无显着差异。高 MAGE-D4 肿瘤中肿瘤细胞的增殖活性显着较高（平均增殖指数，高 MAGE-D4 肿瘤水平为 58.3，低 MAGE-D4 肿瘤水平为 34.0；p
Purpose: MAGE-D4, originally termed MAGE-E1, is a novel MAGE family gene that is expressed at high levels in malignant tumors as compared to normal tissue. The present study was conducted to assess the clinical significance of MAGE-D4 expression in non-small cell lung cancer (NSCLC).Experimental design: Expression of MAGE-D4 protein was estimated by immunohistochemistry and MAGE-D4 mRNA expression was investigated using quantitative reverse transcription-PCR (RT-PCR).Results: We assessed MAGE-D4 expression in NSCLC tissues and was found to be up-regulated in tumor tissues compared with normal lung tissues (mean MAGE-D4/GAPDH values, 0.035 for tumor tissues and 0.009 for normal lung tissues; p=0.002). However, there was no significant difference in MAGE-D4 expression among different pathological stages. The proliferative activity of tumor cells was significantly higher in high MAGE-D4 tumors (mean proliferative indices, 58.3 for high MAGE-D4 tumor levels and 34.0 for low MAGE-D4 tumor levels; p