Immunomagnetic separation: An effective pretreatment technology for isolation and enrichment in food microorganisms detection.
Immunomagnetic separation: An effective pretreatment technology for isolation and enrichment in food microorganisms detection.
复制标题
免疫磁分离：一种有效的食品微生物分离富集前处理技术。
DOI：
10.1111/1541-4337.12656
复制
发表时间：
2020-10
影响因子：
14.8
通讯作者：
Zhouli Wang;Rui Cai;Zhenpeng Gao;Yahong Yuan;T. Yue
中科院分区：
文献类型：
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作者：
Zhouli Wang;Rui Cai;Zhenpeng Gao;Yahong Yuan;T. Yue
高效、准确地检测食品中的食源性病原体和腐败微生物是一项具有重大社会、经济和公共卫生重要性的任务。然而，食品样品中目标细菌的污染水平非常低。由于食品成分的背景干扰和非目标菌群的负面影响，建立高效的前处理技术对于食品微生物的检测至关重要。基于免疫磁性颗粒（IMPs）的免疫磁性分离（IMS）分析具有特异性高、分离效率高等显着优点，被认为是分离和富集目标细菌的强大系统。本文主要介绍IMS的发展及其在食品微生物检测中的应用。首先介绍了IMS在食品细菌浓缩中的基本原理。其次，讨论了不同因素，包括磁性颗粒（MP）的大小、抗体的固定和操作参数（抗体与MP的摩尔比、IMP的量、孵育时间和细菌浓度）对IMP的免疫捕获效率的影响。还评估了 IMP 在不同食品样品中的性能。最后，总结了IMS与各种检测方法（基于免疫学的方法、基于核酸的方法、荧光方法和生物传感器）相结合来检测致病菌和腐败菌。还提出了IMS面临的挑战和未来趋势。 IMS作为一种有效的前处理技术，可以提高检测灵敏度，缩短检测时间，在食品细菌检测领域展现出广阔的前景。
The high efficiency and accurate detection of foodborne pathogens and spoilage microorganisms in food are a task of great social, economic, and public health importance. However, the contamination levels of target bacteria in food samples are very low. Owing to the background interference of food ingredients and negative impact of nontarget flora, the establishment of efficient pretreatment techniques is very crucial for the detection of food microorganisms. With the significant advantages of high specificity and great separation efficiency, immunomagnetic separation (IMS) assay based on immunomagnetic particles (IMPs) has been considered as a powerful system for the separation and enrichment of target bacteria. This paper mainly focuses on the development of IMS as well as their application in food microorganisms detection. First, the basic principle of IMS in the concentration of food bacteria is presented. Second, the effect of different factors, including the sizes of magnetic particles (MPs), immobilization of antibody and operation parameters (the molar ratio of antibody to MPs, the amount of IMPs, incubation time, and bacteria concentration) on the immunocapture efficiency of IMPs are discussed. The performance of IMPs in different food samples is also evaluated. Finally, the combination of IMS and various kinds of detection methods (immunology-based methods, nucleic acid-based methods, fluorescence methods, and biosensors) to detect pathogenic and spoilage organisms is summarized. The challenges and future trends of IMS are also proposed. As an effective pretreatment technique, IMS can improve the detection sensitivity and shorten their testing time, thus exhibiting broad prospect in the field of food bacteria detection.