EFFECT OF RADIATION AND FREEZING ON [H-3] DNA OF YERSINIA-ENTEROCOLITICA
EFFECT OF RADIATION AND FREEZING ON [H-3] DNA OF YERSINIA-ENTEROCOLITICA
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DOI：
10.1128/aem.47.5.1101-1105.1984
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发表时间：
1984-01-01
影响因子：
4.4
通讯作者：
ELZAWAHRY, YA
中科院分区：
文献类型：
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作者：
GRECZ, N;ELZAWAHRY, YA
肠道致病菌Y.小肠结肠炎菌至-18和-75 °。C分别引起7和42%的细胞死亡，并且分别引起0.329和0.588个单链断裂/108道尔顿的DNA;辐射至1D 10（10%细胞存活）与冷冻至2 ℃结合。0 °，-18.degree.和-75 °。C分别诱导0.05、0.75和5.04个单链断裂。相对于在-18 ℃下单链断裂的产率，辐射有效性的增加。和-75 °。C与预期相反，似乎是由于这些低温阻止了单链断裂的修复。
Freezing of the enteropathogenic bacterium Y. enterocolitica to -18.degree. and -75.degree. C caused 7 and 42% cell death, respectively, and 0.329 and 0.588 single-strand breaks/108 daltons of DNA, respectively; radiation to 1 D10 (10% cell survival) combined with freezing to 2.degree.-0.degree., -18.degree. and -75.degree. C induced 0.05, 0.75 and 5.04 single-strand breaks, respectively. The increase in the effectiveness of radiation with respect to the yield of single-strand breaks at -18.degree. and -75.degree. C is contrary to expectation and seems to be due to arrest of repair of single-strand breaks by these low temperatures.