Demonstration of glucuronic acid on brain glycoproteins which react with HNK-1 antibody.
Demonstration of glucuronic acid on brain glycoproteins which react with HNK-1 antibody.
复制标题
演示脑糖蛋白上的葡萄糖醛酸与 HNK-1 抗体发生反应。
DOI：
10.1016/s0006-291x(86)80581-2
复制
发表时间：
1986
影响因子：
3.1
通讯作者：
Jungalwala,FB
中科院分区：
文献类型：
--
作者：
Shashoua,VE;Daniel,PF;Moore,ME;Jungalwala,FB
室管膜蛋白是金鱼和哺乳动物脑细胞外糖蛋白的一个家族，含有N-连接的复合聚糖链。这些糖蛋白与单克隆抗体HNK-1反应，HNK-1识别人淋巴细胞亚群上的膜抗原、髓鞘相关糖蛋白（MAG）和几种神经细胞粘附分子（N-CAM）。先前显示与HNK-1抗体反应的碳水化合物表位包括存在于神经组织的某些糖脂中的末端3-磺基葡糖醛酸基残基（Chou等人，生物化学、生物物理通信资源1985，128，383-388）。在这份报告中，葡萄糖醛酸的存在室管膜素证明了气液色谱和质谱。我们认为3-磺基葡萄糖醛酸残基可能是HNK-1反应性糖蛋白的共同表位。
Ependymins, a family of extracellular glycoproteins of goldfish and mammalian brain, were shown to contain N-linked complex glycan chains. These glycoproteins reacted with a monoclonal antibody, HNK-1 which recognizes a membrane antigen on a subset of human lymphocytes, myelin-associated glycoprotein (MAG) and several neural cell adhesion molecules (N-CAM). The carbo-hydrate epitope reacting with HNK-1 antibody was previously shown to include a terminal 3-sulfoglucuronosyl residue present in certain glycolipids of the nervous tissue (Chou et al., Biochem. Biophys. Res. Commun. 1985,128, 383–388). In this report, the presence of glucuronic acid in ependymins was demonstrated by gas-liquid chromatography and mass spectrometry. We suggest that a 3-sulfoglucuronosyl residue may be the common epitope on HNK-1-reactive glycoproteins.