Evaluating the ability of the Barley stripe mosaic virus-induced gene silencing system to simultaneously silence two wheat genes
Evaluating the ability of the Barley stripe mosaic virus-induced gene silencing system to simultaneously silence two wheat genes
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DOI：
10.1556/crc.36.2008.suppl.b.18
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发表时间：
2008-01-01
影响因子：
1.6
通讯作者：
Scofield, Steven R.
中科院分区：
文献类型：
--
作者：
Cakir, Cahid;Scofield, Steven R.
病毒诱导的基因沉默（VIGS）是快速评价平原地区基因功能的重要工具。通过比较小麦植物感染带有顺式连接的PDS和SGT 1基因的178 bp片段的BSMV-VIGS构建体后小麦PDS和SGT 1基因的下调程度，评估了大麦条纹花叶病毒（BSMV）VIGS系统同时沉默两个基因的能力。或感染单-基因沉默BSMV构建体，其携带单独的PDS或SGT 1或与单基因构建体的混合物。虽然在这些研究中发生了显着的变化，它清楚地表明，两个小麦基因可以同时沉默一个单一的BSMV-VIGS构建体。
Virus-induced gene silencing (VIGS) is an important tool for rapid assessment of gene function in plains. The ability of the Barley stripe mosaic virus (BSMV) VIGS system to simultaneously silence two genes was assessed by comparing the extent of down-regulation of the wheat PDS and SGT1 genes after wheat plants were infected with BSMV-VIGS constructs carrying 178bp fragments of the PDS and SGT1 genes linked in cis or after infection with single-gene silencing BSMV constructs carrying PDS or SGT1 alone or with a mixture of the single-gene constructs. Although significant variation occurred in these studies it was clearly demonstrated that two wheat genes can be silenced simultaneously by a single BSMV-VIGS construct.