Synergistic effect of paeonol and cisplatin on oesophageal cancer cell lines
Synergistic effect of paeonol and cisplatin on oesophageal cancer cell lines
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丹皮酚和顺铂对食管癌细胞系的协同作用。
DOI：
10.1016/j.dld.2008.01.012
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发表时间：
2008-07-01
影响因子：
4.5
通讯作者：
Liu, S. -H.
中科院分区：
文献类型：
--
作者：
Wan, X. -A.;Sun, G. -P.;Liu, S. -H.
背景/目标。丹皮酚是来自牡丹皮的酚类成分，在我们之前的研究中显示出巨大的抗肿瘤活性。本研究旨在探讨丹皮酚与顺铂是否具有协同抑制人食管癌细胞系生长的作用及其可能的机制。方法。通过MTT测定来测量细胞活力。通过药物相互作用系数来分析药物-药物相互作用。通过吖啶橙荧光染色和流式细胞术检测细胞凋亡。通过免疫组织化学染色测定Bcl-2、Bax和caspase-3的表达。结果。丹皮酚与不同浓度的顺铂组合后观察到对两种细胞系活力的协同抑制作用。进一步的研究表明，与单独使用丹皮酚或顺铂治疗组相比，该组合诱导了更大的细胞凋亡。各治疗组Bcl-2表达降低，Bax表达升高，尤其联合组，Bcl-2/Bax比值相应降低。与单独使用任一药物相比，该组合还导致 caspase-3 更大的激活。结论。丹皮酚与顺铂联用对食管细胞系具有显着的协同生长抑制作用，其可能与诱导细胞凋亡有关。 (c) 2008 年 Editrice Gastroenterologica Italiana S.r.l.由爱思唯尔有限公司出版。保留所有权利。
Background/aim. Paeonol, a phenolic component from the root bark of Paeonia moutan, has shown great promise in antitumour activities in our previous studies. The present study was designed to investigate whether paeonol has synergistic effect with cisplatin on the growth-inhibitory of human oesophageal cancer cell lines and the possible mechanism.Methods. Cell viabililty was measured by MTT assay. Drug-drug interactions were analysed by the coefficient of drug interaction. Apoptosis was detected by acridine orange fluorescence staining and flow cytometry assay. Bcl-2, Bax and caspase-3 expression was assayed by immunohistochemical staining.Results. A synergistic inhibitory effect on viability of the two cell lines was observed after combination of paeonol with various concentrations of cisplatin. Further study showed the combination induced greater apoptosis than the groups treated with paeonol or cisplatin alone. The expression of Bcl-2 was decreased and that of Bax was increased in treatment groups, especially in the combination group, with the ratio of Bcl-2/Bax decreased correspondingly. And the combination also resulted in greater activation of caspase-3 than did either agent alone.Conclusions. Paeonol, in combination with cisplatin, had a significantly synergistic growth-inhibitory effect on oesophageal cell line, which may be related to apoptosis induction. (c) 2008 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd. All rights reserved.