MitoCeption as a new tool to assess the effects of mesenchymal stem/stromal cell mitochondria on cancer cell metabolism and function.
MitoCeption as a new tool to assess the effects of mesenchymal stem/stromal cell mitochondria on cancer cell metabolism and function.
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DOI：
10.1038/srep09073
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发表时间：
2015-03-13
影响因子：
4.6
通讯作者：
Vignais ML
中科院分区：
文献类型：
--
作者：
Caicedo A;Fritz V;Brondello JM;Ayala M;Dennemont I;Abdellaoui N;de Fraipont F;Moisan A;Prouteau CA;Boukhaddaoui H;Jorgensen C;Vignais ML
线粒体的活动是组织动态平衡的核心。线粒体功能障碍构成了许多遗传性疾病的标志，并在肿瘤进展中起着关键作用。线粒体的基本作用，加上它们最近被证明的在细胞之间转移的能力，显然有助于他们目前的兴趣。然而，由于缺乏合适的实验工具，确定线粒体在特定生物环境中的适当作用受到了阻碍。我们设计了一种将从A型细胞分离的线粒体直接定量转移到B型受体细胞的方案(MitoCept)。我们通过成像、荧光激活细胞分选(FACS)和线粒体DNA分析验证和定量了有效的线粒体转移。我们发现，微量的间充质干细胞/基质细胞(MSC)线粒体转移到癌细胞，这是一个在共培养中自然发生的过程，导致癌细胞氧化磷酸化(OXPHOS)活性增强，并有利于癌细胞的增殖和侵袭。因此，可以应用于不同细胞系统的有丝分裂感受器技术将成为分析外源线粒体在宿主细胞中诱导的代谢修饰的一种选择方法。
Mitochondrial activity is central to tissue homeostasis. Mitochondria dysfunction constitutes a hallmark of many genetic diseases and plays a key role in tumor progression. The essential role of mitochondria, added to their recently documented capacity to transfer from cell to cell, obviously contributes to their current interest. However, determining the proper role of mitochondria in defined biological contexts was hampered by the lack of suitable experimental tools. We designed a protocol (MitoCeption) to directly and quantitatively transfer mitochondria, isolated from cell type A, to recipient cell type B. We validated and quantified the effective mitochondria transfer by imaging, fluorescence-activated cell sorting (FACS) and mitochondrial DNA analysis. We show that the transfer of minute amounts of mesenchymal stem/stromal cell (MSC) mitochondria to cancer cells, a process otherwise occurring naturally in coculture, results in cancer cell enhanced oxidative phosphorylation (OXPHOS) activity and favors cancer cell proliferation and invasion. The MitoCeption technique, which can be applied to different cell systems, will therefore be a method of choice to analyze the metabolic modifications induced by exogenous mitochondria in host cells.
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影响因子：
0.9
作者：
Kitani, T.;Kami, D.;Gojo, S.
通讯作者：
Gojo, S.
影响因子：
4.3
作者：
Bonuccelli, Gloria;Tsirigos, Aristotelis;Lisanti, Michael P.
通讯作者：
Lisanti, Michael P.
影响因子：
56.9
作者：
Ishikawa, Kaori;Takenaga, Keizo;Hayashi, Jun-Ichi
通讯作者：
Hayashi, Jun-Ichi
影响因子：
7.5
作者：
Kéramidas M;de Fraipont F;Karageorgis A;Moisan A;Persoons V;Richard MJ;Coll JL;Rome C
通讯作者：
Rome C
影响因子：
3.1
作者：
Liu, Kaiming;Ji, Kunqian;Yan, Chuanzhu
通讯作者：
Yan, Chuanzhu