Morphogenesis of rat experimental pulmonary emphysema induced by intratracheally administered papain:: changes in elastic fibres
Morphogenesis of rat experimental pulmonary emphysema induced by intratracheally administered papain:: changes in elastic fibres
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DOI：
10.14670/hh-21.1309
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发表时间：
2006-12-01
影响因子：
2
通讯作者：
Canteras, M.
中科院分区：
文献类型：
--
作者：
Pastor, Luis Miguel;Sanchez-Gascon, F.;Canteras, M.
弹性纤维在肺气肿肺的超微结构的变化已在男性进行了研究，但在实验性肺气肿动物的这一主题的工作很少存在。本文用光镜和电镜观察了肺气肿的形态发生和木瓜蛋白酶滴注引起的弹性纤维的改变。以3 mg/100 g动物体重的速率通过气管向Wistar大鼠滴注木瓜蛋白酶。分别在酶滴注后12 h、3 d、10 d和60 d处死动物。对对照组和实验组的“平均线性截距”（MLI）、“开窗数/呼吸单位”（NF）、“每mm肺泡壁的巨噬细胞数”（NM）和“每mm（2）呼吸单位数”（RU）进行了研究。治疗后两个月，与对照组相比，实验组显示MLI（p < 0.001）和NF（p < 0.001）显著增加，RU数量减少（p < 0.05）。偏相关分析显示MLI和NF之间仅存在正相关。木瓜蛋白酶滴注后12小时，观察到炎症反应，弹性纤维破裂，而微纤维组分保留。在木瓜蛋白酶滴注后三天观察到新形成的泽兰素弹性纤维。10天后，肺泡间水肿消失，弹性纤维形态正常，但可见不规则的弹性纤维条。观察到全小叶、小叶中心和正常肺区的混合模式。木瓜蛋白酶滴注后两个月，观察到与胶原纤维相关的不规则轮廓的弹性纤维的丰富积累。总之，研究的形态学参数显示肺气肿的显著进展。NF和MLI之间的强相关性表明木瓜蛋白酶诱导的肺气肿主要是由肺泡壁的破坏引起的。结果似乎指向一个非常异常的重塑过程与弹性纤维再生，虽然没有迹象表明这些新的纤维在肺气肿大鼠肺木瓜蛋白酶诱导的破坏。
The ultrastructural changes of elastic fibres in emphysematous lungs have been studied in men, but few works exist on this topic in experimental emphysematous animals. In this paper, the morphogenesis of emphysema and alterations of the elastic fibres produced by the instillation of papain are described by light and electron microscopy. Wistar rats were instilled through the trachea with papain at a rate of 3 mg/100 g animal weight. The animals were sacrificed 12 h, 3 days, 10 days and 60 days after enzyme instillation. The "Mean Linear Intercept" (MLI), the "Number of fenestrations/respiratory units" (NF) the "Number of macrophages per mm of alveolar wall" (NM) and the "Number of respiratory unit/mm(2)" (RU), both in the control and experimental groups were studied. Two months after treatment, the experimental group showed a strong increase in the MLI (p < 0.001) and NF (p < 0.001), and a diminished number of RU (p < 0.05) compared with the control group. Partial correlation analysis showed a positive correlation only between MLI and NF. Twelve hours after papain instillation an inflammatory response was observed, the elastic fibres were ruptured, while the microfibrilar component remained. New formations of eulanin elastic fibres were observed three days post papain instillation. After ten days the interalveolar oedema had disappeared and the elastic fibres were of normal morphology although irregular groups of strips of elastic fibres were evident. A mixed pattern of panlobular, centrilobular and normal lung zones were observed. Two months after papain instillation abundant accumulations of elastic fibres of irregular outline were observed associated to collagen fibres. In conclusion, the morphometric parameters studied showed a significant progression of the emphysema. The strong correlation between NF and MLI suggested that papain-induced emphysema is principally caused by breaches of the alveolar walls. The results seem to point to a very abnormal remodelling process associated with elastic fibre regeneration, although there were no signs of destruction of these new fibres formed in emphysematous rat lung induced by papain.