Effects of growth hormone, melatonin, oestrogens and phytoestrogens on the oxidized glutathione (GSSG)/reduced glutathione (GSH) ratio and lipid peroxidation in aged ovariectomized rats
Effects of growth hormone, melatonin, oestrogens and phytoestrogens on the oxidized glutathione (GSSG)/reduced glutathione (GSH) ratio and lipid peroxidation in aged ovariectomized rats
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DOI：
10.1007/s10522-010-9282-7
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发表时间：
2010-12-01
期刊：
影响因子：
4.5
通讯作者：
De la Fuente, Monica
中科院分区：
文献类型：
--
作者：
Baeza, Isabel;Fdez-Tresguerres, Jesus;De la Fuente, Monica
卵巢切除术是人类绝经期常用的大鼠和小鼠模型。卵巢切除术后，氧化剂产生和抗氧化剂水平之间的不平衡似乎有利于前者，增加氧化应激，从而加速衰老。在目前的工作中，还原型谷胱甘肽（GSH），相关的抗氧化剂，氧化型谷胱甘肽（GSSG），氧化剂化合物，以及脂质过氧化（通过丙二醛（MDA）水平）的水平，在肝脏，心脏，肾脏和脾脏匀浆的老年（24个月的年龄）unovariectomized和ovariectomized雌性Wistar大鼠进行了研究。结果表明，与unovariectomized动物相比，卵巢切除大鼠的所有研究器官中的GSSG/GSH比值（氧化应激的标志物）显著增加，MDA产生量也更高。这些数据证实了卵巢切除术加速衰老过程的观点。生长激素（GH），褪黑激素（MEL）和雌激素（OE），以及大豆植物雌激素（PE）的管理10周，年龄在22和24个月之间，能够减少在卵巢切除老年大鼠的研究器官的氧化应激，从而减缓这些动物的衰老过程。
Ovariectomy constitutes a commonly used model in rats and mice for human menopause. After ovariectomy, an imbalance between oxidant production and antioxidant levels appears in favour of the former, with increased oxidative stress and consequently an acceleration of ageing. In the present work, the levels of reduced glutathione (GSH), a relevant antioxidant, and oxidized glutathione (GSSG), an oxidant compound, as well as lipid peroxidation (through malondialdehyde (MDA) levels), were studied in liver, heart, kidney and spleen homogenates of old (24 months of age) unovariectomized and ovariectomized female Wistar rats. The results showed a significant increase of the GSSG/GSH ratio, a marker of oxidative stress, and higher MDA production in all the studied organs of ovariectomized rats as compared with unovariectomized animals. These data confirm the idea that ovariectomy accelerates the ageing process. Administration of growth hormone (GH), melatonin (MEL) and oestrogens (OE), as well as soybean phytoestrogens (PE) for 10 weeks, between 22 and 24 months of age, was able to decrease oxidative stress in the investigated organs of ovariectomized old rats, therefore slowing down the ageing process in those animals.