Participation of c-myc protein in DNA synthesis of human cells.
Participation of c-myc protein in DNA synthesis of human cells.
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c-myc 蛋白参与人体细胞 DNA 合成。
DOI：
10.1126/science.3532322
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发表时间：
1986
期刊：
影响因子：
56.9
通讯作者：
R. Watt
中科院分区：
文献类型：
--
作者：
G. P. Studzinski;Z. Brelvi;S. C. Feldman;R. Watt
癌基因c-myc的蛋白产物被认为在细胞周期的调节中是重要的。然而，它在DNA合成中的直接作用尚未被探索。这里提出的实验表明，除了亲和纯化的抗人c-myc蛋白的抗体，从几种类型的人细胞分离的细胞核可逆地抑制这些细胞核的DNA合成和DNA聚合酶活性。这表明c-myc编码的蛋白质在功能上参与DNA合成。
The protein product of oncogene c-myc is believed to be important in regulation of the cell cycle. However, its direct role in DNA synthesis has not been explored. Experiments presented here show that the addition of affinity-purified antibodies against the human c-myc protein to nuclei isolated from several types of human cells reversibly inhibited DNA synthesis and DNA polymerase activity of these nuclei. This suggests that c-myc encodes a protein that is functionally involved in DNA synthesis.