Functional analysis of ZmCOP1 and ZmHY5 reveals conserved light signaling mechanism in maize and Arabidopsis
Functional analysis of ZmCOP1 and ZmHY5 reveals conserved light signaling mechanism in maize and Arabidopsis
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ZmCOP1 和 ZmHY5 的功能分析揭示了玉米和拟南芥中保守的光信号机制
DOI：
10.1111/ppl.13099
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发表时间：
2020-04-05
影响因子：
6.4
通讯作者：
Lin, Rongcheng
中科院分区：
文献类型：
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作者：
Huai, Junling;Jing, Yanjun;Lin, Rongcheng
植物已经进化出光信号传导机制，以最佳地适应环境光环境的发育模式。组成型光形态发生蛋白1（COP 1）和长下胚轴5（HY 5）是拟南芥光信号转导途径中的两个关键组分。COP 1作为E3泛素连接酶，靶向阳性调节因子，如HY 5，导致它们在黑暗中降解。然而，玉米（Zea mays）中COP 1-HY 5模块的功能分析尚未报道。在这里，我们研究了表达模式和作用的COP 1和HY 5的直系同源物，ZmCOP 1和ZmHY 5，在调节光形态建成。这两个基因与拟南芥同源基因具有高度的氨基酸同源性，并且都受光的调控。亚细胞定位实验表明，ZmCOP 1定位于胞浆，ZmHY 5定位于细胞核。ZmCOP 1的外源表达挽救了cop 1 -4突变体的生理缺陷，ZmHY 5的表达补充了拟南芥中hy 5 -215突变体的长下胚轴表型。酵母双杂交和荧光共振能量转移实验表明ZmCOP 1与ZmHY 5相互作用。本研究对玉米和拟南芥中COP 1-HY 5信号通路的保守功能和调控机制进行了深入研究。
Plants have evolved light signaling mechanisms to optimally adapt developmental patterns to the ambient light environments. CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1) and LONG HYPOCOTYL5 (HY5) are two critical components in the light signaling pathway in Arabidopsis thaliana. COP1 acts as an E3 ubiquitin ligase that targets positive regulators, such as HY5, leading to their degradation in darkness. However, functional analysis of the COP1-HY5 module in maize (Zea mays) has not been reported. Here, we investigated the expression patterns and roles of the COP1 and HY5 orthologs, ZmCOP1 and ZmHY5, in regulating photomorphogenesis. These two genes have high amino acid identities with their Arabidopsis homolog and were both regulated by light. Subcellular localization assay showed that ZmCOP1 was distributed in the cytosol and ZmHY5 localized in the nucleus. Exogenous expression of ZmCOP1 rescued the physiological defects of the cop1-4 mutant, and expression of ZmHY5 complemented the long hypocotyl phenotype of the hy5-215 mutant in Arabidopsis. Yeast two-hybrid and fluorescence resonance energy transfer assays showed that ZmCOP1 interacted with ZmHY5. Our study gains insight into the conserved function and regulatory mechanism of the COP1-HY5 signaling pathway in maize and Arabidopsis.