Melatonin Regulatory Mechanisms and Phylogenetic Analyses of Melatonin Biosynthesis Related Genes Extracted from Peanut under Salinity Stress
Melatonin Regulatory Mechanisms and Phylogenetic Analyses of Melatonin Biosynthesis Related Genes Extracted from Peanut under Salinity Stress
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DOI：
10.3390/plants9070854
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发表时间：
2020-07-01
期刊：
影响因子：
4.5
通讯作者：
Omar, Ahmad A.
中科院分区：
文献类型：
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作者：
ElSayed, Abdelaleim, I;Boulila, Moncef;Omar, Ahmad A.
褪黑素通过保护植物细胞免受氧化应激损伤，提高植物对各种环境胁迫的耐受性。本研究的目的是确定外源褪黑激素（MT）处理是否有助于保护花生（arachhis hypogaea）幼苗免受盐胁迫。这是通过研究盐胁迫下的酶促和非酶促抗氧化系统以及褪黑激素生物合成相关基因的表达来实现的。结果表明，盐胁迫下花生幼苗的活性氧（ROS）浓度显著增加。外源褪黑激素通过激活抗氧化酶降低盐胁迫下花生幼苗的ROS水平。盐度胁迫下，150 μ M褪黑素处理可上调褪黑素生物合成相关基因如n -乙酰5-羟色胺甲基转移酶（ASMT1、ASMT2、ASMT3）、色氨酸脱羧酶（TDC）和色胺5-羟化酶（T5H）的转录水平。结果表明，褪黑素通过诱导酶促或非酶促抗氧化系统调节氧化还原稳态。此外，对不同植物的56条褪黑素生物合成基因（ASMT1、ASMT2、ASMT3、TDC、T5H）进行了系统发育分析，其中包括5条新提取的从紫檀中提取的序列。hypogaea)。这是基于配对核苷酸和预测氨基酸之间的两两比较，以及取代率和系统发育推断。分析的序列具有异质性和异质性。次gaeaaccites主要与manihot esculenta最接近，但这需要进一步澄清。
Melatonin improves the tolerance of plants to various environmental stresses by protecting plant cells against oxidative stress damage. The objective of the current study was to determine whether exogenous melatonin (MT) treatments could help protecting peanut (Arachis hypogaea) seedlings against salinity stress. This was achieved by investigating enzymatic and non-enzymatic antioxidant systems and the expression of melatonin biosynthesis related genes in response to salinity stress with or without exogenous MT. The results showed a significant increase in the concentrations of reactive oxygen species (ROS) in peanut seedlings under salinity stress. The exogenous application of melatonin decreased the levels of ROS through the activation of antioxidant enzymes in peanut seedlings under salinity stress. Transcription levels of melatonin biosynthesis related genes such as N-acetylserotonin methyltransferase (ASMT1,ASMT2,ASMT3), tryptophan decarboxylase (TDC), and tryptamine 5-hydroxylase (T5H) were up-regulated with a 150 mu M melatonin treatment under salinity stress. The results indicated that melatonin regulated the redox homeostasis by its ability to induce either enzymatic or non-enzymatic antioxidant systems. In addition, phylogenetic analysis of melatonin biosynthesis genes (ASMT1,ASMT2,ASMT3,TDC,T5H) were performed on a total of 56 sequences belonging to various plant species including five new sequences extracted fromArachis hypogaea(A. hypogaea). This was based on pairwise comparison among aligned nucleotides and predicted amino acids as well as on substitution rates, and phylogenetic inference. The analyzed sequences were heterogeneous and theA. hypogaeaaccessions were primarily closest to those ofManihot esculenta, but this needs further clarification.