Formation of Phosphatidylethanol and Its Subsequent Elimination During an Extensive Drinking Experiment Over 5 Days
Formation of Phosphatidylethanol and Its Subsequent Elimination During an Extensive Drinking Experiment Over 5 Days
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DOI：
10.1111/j.1530-0277.2012.01768.x
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发表时间：
2012-09-01
影响因子：
3.2
通讯作者：
Thierauf, Annette
中科院分区：
文献类型：
--
作者：
Gnann, Heike;Weinmann, Wolfgang;Thierauf, Annette
背景近30年来，磷脂酰乙醇酯(Peth)一直被认为是饮酒的直接标志。这个标记物代表大量和较长时间的摄入，但也在单次摄入乙醇(Etoh)1次后被检测到。本研究的目的是通过模拟大量饮酒来进一步了解peth 16：0/18：1的形成和消除。方法在戒酒3周后，11名受试者连续5天每天饮用一定量的乙醇，血液酒精浓度估计为1g/kg。在饮酒后，他们禁食了16天，并定期抽血。PEH 16：0/18：1分析采用高效液相色谱-串联质谱仪(高效液相色谱1100系统)和QTrap 2000三重四极线离子陷阱质谱仪。采用顶空气相色谱火焰离子化检测器标准化方法测定血液中的酒精含量。结果乙醇的最高检测浓度为0.99~1.83g/kg，平均为1.32g/kg。这些值在开始饮酒后1-3小时(平均1.9小时)达到。作为对照，11名受试者中有10名在饮酒后1h可检测到聚乙醇胺16：0/18：1，范围为45~138 ng/mlpeth 16：0/18：1。在接下来的几天里，聚乙醇胺16：0/18：1浓度持续升高，在第3~6天达到最高浓度74~237 ng/ml。然而，与先前研究中的酒精滥用者相比，peth 16：0/18：1的浓度保持在相当低的水平。
Background For almost 30years, phosphatidylethanol (PEth) has been known as a direct marker of alcohol consumption. This marker stands for consumption in high amounts and for a longer time period, but it has been also detected after 1 high single intake of ethanol (EtOH). The aim of this study was to obtain further information about the formation and elimination of PEth 16:0/18:1 by simulating extensive drinking. Methods After 3weeks of alcohol abstinence, 11 test persons drank an amount of EtOH leading to an estimated blood ethanol concentration of 1g/kg on each of 5 successive days. After the drinking episode, they stayed abstinent for 16days with regular blood sampling. PEth 16:0/18:1 analysis was performed using liquid chromatography-tandem mass spectrometry (high-performance liquid chromatography 1100 system and QTrap 2000 triple quadrupole linear ion trap mass spectrometer. Values of blood alcohol were obtained using a standardized method with headspace gas chromatography flame ionization detector. Results Maximum measured concentrations of EtOH were 0.99 to 1.83g/kg (mean 1.32g/kg). These values were reached 1 to 3hours after the start of drinking (mean 1.9hours). For comparison, 10 of 11 volunteers had detectable PEth 16:0/18:1 values 1hour after the start of drinking, ranging from 45 to 138ng/ml PEth 16:0/18:1. Over the following days, concentrations of PEth 16:0/18:1 increased continuously and reached the maximum concentrations of 74 to 237ng/ml between days 3 and 6. Conclusions This drinking experiment led to measurable PEth concentrations. However, PEth 16:0/18:1 concentrations stayed rather low compared with those of alcohol abusers from previous studies.