Characterization of TEMINAL FLOWER1 homologs CmTFL1c gene from Chrysanthemum morifolium
Characterization of TEMINAL FLOWER1 homologs CmTFL1c gene from Chrysanthemum morifolium
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DOI：
10.1007/s11103-019-00838-6
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发表时间：
2019-04-01
影响因子：
5.1
通讯作者：
Zhang, Qixiang
中科院分区：
文献类型：
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作者：
Gao, Yaohui;Gao, Yike;Zhang, Qixiang
关键信息菊花CmTFL1c基因抑制开花，调节花序结构和花发育。基因末端FLOWER1(TFL1)参与了分生组织命运的调控和开花时间的调控。在此，从菊花中克隆了一个TFL1基因，命名为CmTFL1c，并对其进行了进一步的鉴定。CmTFL1c的开放阅读框全长522bp，编码173个氨基酸的多肽。系统发育分析表明，CmTFL1c属于CEN/TFL1分支。CmTFL1c蛋白定位于胞核和质膜，提示CmTFL1c可能是一种转录因子。CmTFL1c基因在营养茎中表达最高，在叶片和花蕾中表达较弱，茎尖和茎均对光周期敏感。CmTFL1c在野生拟南芥和tfl1-13突变体中的过表达导致了开花延迟和结构改变，包括增加二次分枝，以及异常的花序和花。CmTFL1c基因通过抑制AtFT、AtLFY和AtAP1基因的上调来负向调控开花。通过农杆菌介导法对CmTFL1c在菊花中的生物学功能进行了进一步研究，结果表明，CmTFL1c不仅延缓了菊花的开花，促进了腋芽的形成，而且在菊花花序的形成中起着重要的作用。这些结果表明，CmTFL1c影响开花时间，调节花序构型。
Key messageThe CmTFL1c gene of Chrysanthemum morifolium inhibits flowering, regulates inflorescence architecture and floral development.AbstractThe timing of flowering is an important ornamental trait of chrysanthemum. The gene TERMINAL FLOWER1 (TFL1) has been shown to be involved in the regulation of meristem fate and flowering time regulation. Here, a TFL1 gene named as CmTFL1c, was cloned from Chrysanthemum morifolium and further characterized. The open reading frame of CmTFL1c comprises 522bp, which encodes a polypeptide of 173 amino acids. Phylogenetic analysis revealed that CmTFL1c belongs to the CEN/TFL1 clade. CmTFL1c protein localizes to the nucleus as well as to plasma membrane, which suggests that CmTFL1c may be a transcription factor. The CmTFL1c gene was most highly expressed in vegetative stems, and weakly expressed in leaves and flower buds; both shoot apices and stems had sensitivity to photoperiod. Overexpression of CmTFL1c in wild Arabidopsis and tfl1-13 mutant led to late flowering and altered architecture, including increased secondary branching, and abnormal inflorescences and flowers. The CmTFL1c gene negatively regulated flowering by inhibiting the up-regulation of the AtFT, AtLFY and AtAP1. The biological function of CmTFL1c was further characterized in C. morifolium via Agrobacterium-mediated transformation, which showed that CmTFL1c not only delayed flowering and promoted axillary bud formation, but also played an important role in inflorescence formation of chrysanthemum. These results showed that the CmTFL1c affects flowering time and regulates inflorescence architecture.