An efficient cumate-inducible system for procyclic and bloodstream form Trypanosoma brucei
An efficient cumate-inducible system for procyclic and bloodstream form Trypanosoma brucei
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DOI：
10.1016/j.molbiopara.2017.04.007
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发表时间：
2017-06-01
影响因子：
1.5
通讯作者：
He, Cynthia Y.
中科院分区：
文献类型：
--
作者：
Li, Feng-Jun;Xu, Zhi-Shen;He, Cynthia Y.
In Trypanosoma brucei, the tetracycline-inducible system enables tightly-regulated, highly-efficient expression of recombinant proteins or double-stranded RNA in both procyclic and bloodstream form cells, providing useful molecular genetic tools to study gene functions. An alternative, vanillic acid-inducible system is recently described for procyclic T. brucei, providing similar to 18-fold increase in GFP reporter expression upon induction (Sunter JD. Mol. Biochem. Parasitol. 2016, 207:45-48). Here we describe a cumate-inducible system that allows efficient, tunable gene expression showing > 300-fold increase in GFP expression upon induction. The cumate-inducible system can be used alone or together with the tetracycline-inducible system, in both procyclic and bloodstream form T. brucei Efficient cumate-inducible expression is also achieved in T. brucei-infected mice.
