Variation in rDNA ITS sequences in Glomus mosseae and Gigaspora margarita spores from a permanent pasture
Variation in rDNA ITS sequences in Glomus mosseae and Gigaspora margarita spores from a permanent pasture
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来自永久牧场的 Mosseae 和 Gigaspora margarita 孢子中 rDNA ITS 序列的变异
DOI：
10.1017/s0953756299001860
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发表时间：
2000
期刊：
影响因子：
2.5
通讯作者：
Sarah E. Smith
中科院分区：
文献类型：
--
作者：
Z. Antoniolli;D. Schachtman;K. Ophel;Sarah E. Smith
为了研究Glomus mosseae和Gigaspora margarita的遗传变异性，分析了从永久牧场和盆栽中收集的孢子的rDNA ITS区的序列相似性。用引物ITS 1和ITS 4进行PCR扩增，并对产物进行克隆和测序。从G. mosseae和Gi. margarita的研究证实，在单个孢子中ITS区域存在变异。从两个物种的序列表型分析支持的形态学鉴定，将该物种分为两个独立的组。通过对这些序列的分析，得出了遗传多样性的估计，清楚地表明，G。mosseae的遗传多样性至少是Gigaspora margarita的单个孢子（田间）和一个孢子池（盆栽）的2-5倍。这表明该自然群体中存在着高度的变异。苔藓。
To study the genetic variability in Glomus mosseae and Gigaspora margarita sequence similarities of ITS regions of rDNA were analysed from spores collected from a permanent pasture and from pot cultures. PCR amplification with the primers ITS1 and ITS4 was performed and products were cloned and sequenced. The sequences from single spores of G. mosseae and Gi. margarita confirmed that there is variation in the ITS region in a single spore. Phenetic analysis of sequences from both species supported the morphological identification which placed the species into two separate groups. Through the analysis of these sequences an estimate of genetic diversity was derived which clearly showed that the three field spores of G. mosseae were at least 2–5 times more genetically diverse than one single spore (field) and a pool of spores (pot culture) of Gigaspora margarita. This demonstrates that a high degree of variation exists in this natural population of G. mosseae.