Diagnostic and prognostic value of plasma microRNA-195 in patients with non-small cell lung cancer.
Diagnostic and prognostic value of plasma microRNA-195 in patients with non-small cell lung cancer.
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DOI：
10.1186/s12957-016-0980-8
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发表时间：
2016-08-24
影响因子：
3.2
通讯作者：
Hao G
中科院分区：
文献类型：
--
作者：
Su K;Zhang T;Wang Y;Hao G
最近，循环microRNA（miRNA）已被报道在血浆/血清中可稳定检测，并在各种癌症中用作有效的生物标志物。本研究旨在检测血浆miRNA-195在非小细胞肺癌（NSCLC）患者中的表达水平，探讨其在NSCLC诊断和预后判断中的价值。进行定量实时PCR以评估100名NSCLC患者和100名健康志愿者的血浆miRNA-195水平。分析miRNA-195表达与临床病理因素及总生存期的关系。采用受试者工作特征（ROC）曲线分析法评价血浆miRNA-195对NSCLC诊断的潜在价值。NSCLC患者血浆miRNA-195水平较健康对照组明显下调（P < 0.001）。血浆miRNA-195表达降低与淋巴结转移和晚期临床分期显著相关。ROC曲线分析显示，血浆miRNA-195是NSCLC诊断的有用指标。多变量考克斯回归分析证实血浆miRNA-195低表达是NSCLC患者的独立不利预后因素。这些结果表明，血浆miRNA-195可能作为一个有前途的生物标志物，对NSCLC的早期检测和预后评估。
Recently, circulating microRNAs (miRNAs) have been reported to be stably detectable in plasma/serum and to function as potent biomarkers in various cancers. The aim of this study was to evaluate the expression level of plasma miRNA-195 in patients with non-small cell lung cancer (NSCLC) and investigate its diagnostic and prognostic value. Quantitative real-time PCR was performed to evaluate plasma miRNA-195 levels in 100 NSCLC patients and 100 healthy volunteers. The association between miRNA-195 expression and clinicopathological factors as well as the overall survival was analyzed. Receiver-operating characteristic (ROC) curve analysis was carried out to assess the potential value of plasma miRNA-195 for NSCLC diagnosis. Plasma miRNA-195 was downregulated in NSCLC patients compared with healthy controls (P < 0.001). Decreased plasma miRNA-195 expression was significantly associated with lymph node metastasis and advanced clinical stage. ROC curve analysis showed that plasma miRNA-195 was a useful marker for NSCLC diagnosis. Multivariate Cox regression analysis confirmed low plasma miRNA-195 expression as an independent unfavorable prognostic factor for NSCLC patients. These findings indicate that plasma miRNA-195 might serve as a promising biomarker for the early detection and prognosis evaluation of NSCLC.