Seroprevalence of MERS-CoV in healthy adults in western Saudi Arabia, 2011-2016
Seroprevalence of MERS-CoV in healthy adults in western Saudi Arabia, 2011-2016
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DOI：
10.1016/j.jiph.2020.01.001
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发表时间：
2020-05-01
影响因子：
6.7
通讯作者：
Hashem, Anwar M.
中科院分区：
文献类型：
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作者：
Degnah, Afnan A.;Al-Amri, Sawsan S.;Hashem, Anwar M.
背景：中东呼吸综合征冠状病毒（MERS-CoV）是一种新发现的人畜共患冠状病毒。目前的证据证实了单峰骆驼在人类原发性感染中的作用，但不能解释散发的社区病例。然而，无症状或亚临床病例可能是社区中可能的感染源。方法：从沙特阿拉伯西部50个不同民族的健康成年献血者中收集2011年至2016年期间存档的人血清（7461），用于MERS CoV血清阳性率调查。采用ELISA检测MERS-CoV S1特异性抗体（Abs），并采用假型和活病毒中和试验检测中和Abs（nAbs）。结果：在7461份样本中，来自18个不同民族的174份血清ELISA阳性（2.3%，95%CI 2.0-2.7）。在17份样本（0.23%，95% CI 0.1-0.4）中确认了nAb的存在，其中1份样本在两种中和试验中均显示阳性。确认血清阳性被确定在年轻（15-44岁）的男性和女性从沙特阿拉伯，埃及，也门，巴基斯坦，巴勒斯坦，苏丹和印度没有显着preference.Conclusions：中东呼吸综合征冠状病毒血清阳性率的增加趋势，观察在沙特阿拉伯西部的一般人群，这表明，无症状或轻度感染可能存在，并作为一个未被识别的感染源。来自不同国籍的个体的血清阳性强调了中东呼吸综合征向阿拉伯半岛以外地区输出的可能性。因此，加强和持续监测是非常必要的。(C)2020由Elsevier Ltd代表沙特国王本阿卜杜勒阿齐兹健康科学大学出版。
Background: The Middle East respiratory syndrome coronavirus (MERS-CoV) is a newly recognized zoonotic coronavirus. Current evidence confirms the role of dromedaries in primary human infections but does not explain the sporadic community cases. However, asymptomatic or subclinical cases could represent a possible source of infection in the community.Methods: Archived human sera (7461) collected between 2011 and 2016 from healthy adult blood donors from 50 different nationalities in the western part of Saudi Arabia were obtained for MERS-CoV seroprevalence investigation. Samples were tested for MERS-CoV S1-specific antibodies (Abs) by ELISA and confirmed by testing for neutralizing Abs (nAbs) using both pseudotyped and live virus neutralization assays.Results: Out of 7461 samples, 174 sera from individuals with 18 different nationalities were ELISA positive (2.3%, 95% CI 2.0-2.7). Presence of nAbs was confirmed in 17 samples (0.23%, 95% CI 0.1-0.4) of which one sample exhibited positivity in both neutralization assays. Confirmed seropositivity was identified in young (15-44 years) men and women from Saudi Arabia, Egypt, Yemen, Pakistan, Palestine, Sudan, and India without significant preference.Conclusions: An increasing trend of MERS-CoV seroprevalence was observed in the general population in western Saudi Arabia, suggesting that asymptomatic or mild infections might exist and act as an unrecognized source of infection. Seropositivity of individuals from different nationalities underscores the potential MERS exportation outside of the Arabian Peninsula. Thus, enhanced and continuous surveillance is highly warranted. (C) 2020 Published by Elsevier Ltd on behalf of King Saud Bin Abdulaziz University for Health Sciences.