Neurotranscriptome profiles of multiple zebrafish strains.
Neurotranscriptome profiles of multiple zebrafish strains.
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多种斑马鱼品系的神经转录组图谱。
DOI：
10.1016/j.gdata.2015.06.004
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发表时间：
2015-09-01
期刊：
影响因子：
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通讯作者：
Godwin J
中科院分区：
文献类型：
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作者：
Wong RY;Godwin J
在生物体进化史的背景下，行为表现或生理反应往往受到内在和外在机制的影响。了解转录组的差异可以洞察适应性或病理反应。我们利用高通量测序(RNA测序)来表征四个品系斑马鱼(Danio Rerio)雄性和雌性的神经转录组图谱。菌株因先前记录的压力和焦虑样行为反应的差异，以及野生捕获个体代代相传而有所不同。在这里，我们描述了在生成原始RNA测序读数时的详细方法和质量控制，这些读数可以在NCBI的基因表达总览数据库(GSE61108)中公开获得。
Behavioral displays or physiological responses are often influenced by intrinsic and extrinsic mechanisms in the context of the organism's evolutionary history. Understanding differences in transcriptome profiles can give insight into adaptive or pathological responses. We utilize high throughput sequencing (RNA-sequencing) to characterize the neurotranscriptome profiles in both males and females across four strains of zebrafish (Danio rerio). Strains varied by previously documented differences in stress and anxiety-like behavioral responses, and generations removed from wild-caught individuals. Here we describe detailed methodologies and quality controls in generating the raw RNA-sequencing reads that are publically available in NCBI's Gene Expression Omnibus database (GSE61108).