Increased alpha-B-crystallin expression in mammary metaplastic carcinomas
Increased alpha-B-crystallin expression in mammary metaplastic carcinomas
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DOI：
10.1111/j.1365-2559.2011.03882.x
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发表时间：
2011-08-01
期刊：
影响因子：
6.4
通讯作者：
Tse, Gary M.
中科院分区：
文献类型：
--
作者：
Chan, Siu-Ki;Lui, Philip C. W.;Tse, Gary M.
目的：乳腺化生癌是一种罕见的乳腺癌，可能存在诊断困难。最近报道α-B-晶状体蛋白在基底样癌和化生性癌中表达。 方法和结果：评估了 33 例化生性癌、44 例常规高级别癌和 28 例对照间充质梭形细胞肿瘤中 α-B-晶状体蛋白和常规基底样表型标记物 CK5/6、CK14、p63、c-kit 和表皮的表达。通过免疫组织化学检测生长因子受体（EGFR）。 68% 的化生性癌的 α-B-晶状体蛋白染色呈阳性，所有肿瘤细胞成分的细胞质染色均呈阳性。 CK5/6、CK14、p63、c-kit 和 EGFR 染色分别占化生性癌的 43%、68%、45%、21% 和 25%。结合这些标记物，84% 的化生性癌表达 α B-晶状体蛋白或 CK14。相比之下，只有14%（6例）的常规高级别癌和7%（2例）的间质梭形细胞肿瘤表达αB-晶状体蛋白；除一种癌外，所有这些癌均为 ER/PR/HER2 三阴性。 结论：使用 α B-晶状体蛋白诊断化生性癌的敏感性为 68%，特异性为 88%，阳性预测值为 74%，阴性预测值为 85%，准确度为 78%。 α B-晶状体蛋白/CK14 组合的敏感性可提高至 84%。 α-B-晶状体蛋白可用作诊断化生性癌的辅助标志物。
Aims: Mammary metaplastic carcinoma is a rare breast carcinoma, and may present diagnostic difficulty. Alpha-B-crystallin has been recently reported to be expressed in basal-like and metaplastic carcinomas.Methods and results: Thirty-three metaplastic carcinomas, 44 conventional high-grade carcinomas and 28 mesenchymal spindle cell neoplasms as controls were assessed for their expression of alpha B-crystallin and conventional basal-like phenotypic markers CK5/6, CK14, p63, c-kit and epidermal growth factor receptor (EGFR) by immunohistochemistry. Alpha-B-crystallin staining was positive in 68% of the metaplastic carcinomas with cytoplasmic staining in all tumour cell components. CK5/6, CK14, p63, c-kit and EGFR stained 43%, 68%, 45%, 21% and 25% of the metaplastic carcinomas, respectively. Combining these markers, 84% of the metaplastic carcinomas expressed either alpha B-crystallin or CK14. In comparison, only 14% (six cases) of conventional high-grade carcinoma and 7% (two cases) of mesenchymal spindle cell neoplasm expressed alpha B-crystallin; all but one of these carcinomas were ER/PR/HER2 triple-negative.Conclusions: Using alpha B-crystallin for diagnosis of metaplastic carcinoma gives a 68% sensitivity, 88% specificity, 74% positive predictive value, 85% negative predictive value and 78% accuracy. The sensitivity is enhanced to 84% with combinations of alpha B-crystallin/CK14. Alpha-B-crystallin may be used as an adjunct marker in the diagnosis of metaplastic carcinoma.