Site‐specific Protein Cross‐Linking with Genetically Incorporated 3,4‐Dihydroxy‐L‐Phenylalanine

Site‐specific Protein Cross‐Linking with Genetically Incorporated 3,4‐Dihydroxy‐L‐Phenylalanine
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DOI:
10.1002/cbic.200990036
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发表时间:
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期刊:
影响因子:
3.2
通讯作者:
Aiko Umeda;G. N. Thibodeaux;Jie-ping Zhu;YungAh Lee;Z. Zhang
Aiko Umeda;G. N. Thibodeaux;Jie-ping Zhu;YungAh Lee;Z. Zhang
中科院分区:
生物学3区
文献类型:
--
作者:
Aiko Umeda;G. N. Thibodeaux;Jie-ping Zhu;YungAh Lee;Z. Zhang

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现在来看看l - DOPA:化学交联被广泛用于研究蛋白质相互作用。然而,许多交联剂存在低反应性或选择性的问题。利用基因结合的3,4 -二羟基- L -苯丙氨酸实现了位点特异性蛋白交联的高效选择性反应。
Come together right now withL‐DOPA: Chemical cross‐linking is widely used to study protein–protein interactions. However, many cross‐linking agents suffer from low reactivity or selectivity. An efficient and selective reaction of site‐specific protein cross‐linking was achieved using genetically incorporated 3,4‐dihydroxy‐L‐phenylalanine.