Occurrence of sulfonamide and tetracycline-resistant bacteria and resistance genes in aquaculture environment
Occurrence of sulfonamide and tetracycline-resistant bacteria and resistance genes in aquaculture environment
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水产养殖环境中磺胺类、四环素类耐药细菌及耐药基因的存在
DOI：
10.1016/j.watres.2012.02.004
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发表时间：
2012-05-01
期刊：
影响因子：
12.8
通讯作者：
Xu, Lin
中科院分区：
文献类型：
--
作者：
Gao, Panpan;Mao, Daqing;Xu, Lin
调查了天津市水产养殖环境中磺胺类和四环素类抗药性的发生情况及其污染状况。对天津市6个水产养殖场中存在的耐药细菌进行了鉴定，并对相应的耐药基因进行了定量检测。在各种ARG中，磺胺类耐药基因普遍存在，其浓度最高(Sul1/16S rDNA为3.0×10(-5)~3.3×10(-4)，Sul2/16S rDNA为2.0×10(-4)~1.8×10(-3))，这很可能是因为该地区磺胺类药物的使用比四环素类药物更普遍。磺胺甲恶唑耐药菌(63.27%)和四环素耐药菌(57.14%)均以芽孢杆菌为主。在蜡状芽孢杆菌、枯草芽孢杆菌、巨大芽孢杆菌和lwofii不动杆菌中，至少检测到了上述基因中的两个基因(teM、teto、tet、etW、sul1和sul2)，并在蜡样芽孢杆菌中检测到上述基因，表明该地区存在多重耐药现象。Sul1在肠道细菌(如肠球菌)之间的遗传转移和土生菌(如芽孢杆菌)。系统发育分析表明。几株耐药的条件致病菌(如不动杆菌属)在本土细菌中发现，这增加了ARGs对公共卫生的风险。总体而言，这是首次通过对中国北部典型养殖区耐药细菌种类的分析，采用定性和定量相结合的方法对ARGs进行全面调查的研究。(C)2012爱思唯尔有限公司。保留所有权利。
The occurrence of sulfonamide and tetracycline resistance and their pollution profile in the aquaculture environment of Tianjin, northern China, were investigated. The presence of antibiotic-resistant bacteria was identified and the corresponding antibiotic resistance genes (ARGs) were quantified at 6 aquaculture farms in Tianjin. Sulfonamide-resistance genes were prevalent and their concentrations were the highest detected (3.0 x 10(-5) to 3.3 x 10(-4) for sul1/16S rDNA, 2.0 x 10(-4) to 1.8 x 10(-3) for sul2/16S rDNA) among the various ARGs, most likely because the use of sulfonamides is more prevalent than tetracyclines in this area. Bacillus was the most dominant bacterial genus in both sulfamethoxazole resistant bacteria (63.27% of the total resistant bacteria) and tetracycline-resistant bacteria (57.14% of the total resistant bacteria). At least two of those genes (tetM, tetO, tetT, tetW, sul1 and sul2) were detected in the isolates of Bacillus cereus, Bacillus subtilis, Bacillus megaterium and Acinetobacter lwofii, and all of the above genes were detected in B. cereus, suggesting the occurrence of multi-resistance in the studied area. The genetic transfer of sul1 between intestinal bacteria (e.g., Enterococcus spp.) and indigenous bacteria (e.g., Bacillus spp.) was implied by phylogenetic analysis. Several strains of resistant opportunistic pathogens (e.g., Acinetobacter spp.) were found in indigenous bacteria, which increase the risk of ARGs to public health. Overall, this is the first study to comprehensively investigate the antibiotic resistance profile by analyzing the species of antibiotic-resistant bacteria and adopting qualitative and quantitative methods to investigate ARGs at a typical aquaculture area in northern China. (C) 2012 Elsevier Ltd. All rights reserved.