Light induction of a vertebrate clock gene involves signaling through blue-light receptors and MAP kinases
Light induction of a vertebrate clock gene involves signaling through blue-light receptors and MAP kinases
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DOI：
10.1016/s0960-9822(02)00835-7
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发表时间：
2002-05-14
期刊：
影响因子：
9.2
通讯作者：
Sassone-Corsi, P
中科院分区：
文献类型：
--
作者：
Cermakian, N;Pando, MP;Sassone-Corsi, P
光感受与生物钟的信号传导通路还没有被破译。昼夜光感受器的两组突出的候选者是视蛋白（例如，黑视蛋白）和蓝光光感受器（例如，隐花色素）。我们以前已经表明，斑马鱼是一个理想的模式生物，在其中研究昼夜节律调节和光反应的外周组织。在这里，我们使用光响应斑马鱼细胞系Z3来剖析时钟基因zPer2对光的响应。我们表明，MAPK（丝裂原活化蛋白激酶）途径是必不可少的这种反应，虽然其他信号通路也可能发挥作用。此外，zPer2转录响应单色光的作用光谱分析表明，蓝光感光器的参与。Cry1b和Cry3隐花色素构成有吸引力的候选人，在这种情况下的光感受器。我们的研究结果建立了蓝光光感受器，可能隐花色素，和MAPK通路引起光诱导的时钟基因的转录激活之间的联系。
The signaling pathways that couple light photoreception to entrainment of the circadian clock have yet to be deciphered. Two prominent groups of candidates for the circadian photoreceptors are opsins (e.g., melanopsin) and blue-light photoreceptors (e.g., cryptochromes). We have previously showed that the zebrafish is an ideal model organism in which to study circadian regulation and light response in peripheral tissues. Here, we used the light-responsive zebrafish cell line Z3 to dissect the response of the clock gene zPer2 to light. We show that the MAPK (mitogen-activated protein kinase) pathway is essential for this response, although other signaling pathways may also play a role. Moreover, action spectrum analyses of zPer2 transcriptional response to monochromatic light demonstrate the involvement of a blue-light photoreceptor. The Cry1b and Cry3 cryptochromes constitute attractive candidates as photoreceptors in this setting. Our results establish a link between blue-light photoreceptors, probably cryptochromes, and the MAPK pathway to elicit light-induced transcriptional activation of clock genes.