Genome Sequence of Kitasatospora setae NBRC 14216T: An Evolutionary Snapshot of the Family Streptomycetaceae
Genome Sequence of Kitasatospora setae NBRC 14216T: An Evolutionary Snapshot of the Family Streptomycetaceae
复制标题
DOI：
10.1093/dnares/dsq026
复制
发表时间：
2010-12-31
期刊：
影响因子：
4.1
通讯作者：
Fujita, Nobuyuki
中科院分区：
文献类型：
--
作者：
Ichikawa, Natsuko;Oguchi, Akio;Fujita, Nobuyuki
已知Kitasatospora setae NBRC 14216(T) （=KM-6054(T)）产生具有抗滴虫活性的塞他霉素（巴菲霉素B1）。Kitasatospora属与Streptomyces属形态相似，但根据细胞壁组成和16S rDNA序列可将其区分开来。我们确定了K. setae NBRC 14216(T)的全基因组序列，作为链霉菌科中除链霉菌外的第一个全基因组序列。基因组是一条长度为8 783 278 bp、末端倒置重复序列为127 148 bp的单线性染色体，预计编码7569个蛋白质编码基因、9个rRNA操纵子、1个tmRNA和74个tRNA基因。虽然这些特征与链霉菌相似，但在全基因组比较中，直系金缕金与链霉菌的同源基因显示出较小程度的同源性。基于氨基酸序列的多位点系统发育分析明确地将K. setae置于链霉菌属之外。虽然在链霉菌中发现的许多与形态分化相关的基因在直毛k中高度保守，但存在一些差异，如表面活性蛋白AmfS （SapB）类的明显缺失，参与细胞壁合成的旁系成分的拷贝数和变异也存在差异。
Kitasatospora setae NBRC 14216(T) (=KM-6054(T)) is known to produce setamycin (bafilomycin B1) possessing antitrichomonal activity. The genus Kitasatospora is morphologically similar to the genus Streptomyces, although they are distinguishable from each other on the basis of cell wall composition and the 16S rDNA sequence. We have determined the complete genome sequence of K. setae NBRC 14216(T) as the first Streptomycetaceae genome other than Streptomyces. The genome is a single linear chromosome of 8 783 278 bp with terminal inverted repeats of 127 148 bp, predicted to encode 7569 protein-coding genes, 9 rRNA operons, 1 tmRNA and 74 tRNA genes. Although these features resemble those of Streptomyces, genome-wide comparison of orthologous genes between K. setae and Streptomyces revealed smaller extent of synteny. Multilocus phylogenetic analysis based on amino acid sequences unequivocally placed K. setae outside the Streptomyces genus. Although many of the genes related to morphological differentiation identified in Streptomyces were highly conserved in K. setae, there were some differences such as the apparent absence of the AmfS (SapB) class of surfactant protein and differences in the copy number and variation of paralogous components involved in cell wall synthesis.