Heparin decreases ischemia-reperfusion injury in isolated canine gracilis model.
Heparin decreases ischemia-reperfusion injury in isolated canine gracilis model.
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DOI：
10.1001/archsurg.1988.01400280080014
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发表时间：
1988-04
影响因子：
--
通讯作者：
J. Wright;J. C. Kerr;C. Valeri;R. Hobson
中科院分区：
文献类型：
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作者：
J. Wright;J. C. Kerr;C. Valeri;R. Hobson
骨骼肌缺血再灌注损伤的机制仍存在争议。一些研究者已经证明肝素可以改善心、脑和肾组织的缺血性损伤。我们在10只麻醉杂种狗的离体股薄肌模型中研究了肝素钠减少缺血再灌注损伤的能力。一侧股薄肌正常灌注，对侧股薄肌缺血6小时，再灌注1小时。5只犬接受缺血前肝素钠推注（200 U/kg，静脉内），随后连续输注（15 U/kg/h，静脉内），5只对照犬未接受肝素。骨骼肌缺血再灌注损伤的定量测定采用氯化三苯基四氮唑组织化学染色和梗死面积的计算机面积测量法。来自缺血肌肉的结果证明肝素化的显著有益效果。非肝素化犬的梗死面积为72% ± 5%，肝素化犬的梗死面积显著降低至24% ± 8%。这种保护作用的机制可能是由于肝素的抗凝血、抗血小板或抗炎作用。
The mechanisms of ischemia-reperfusion injury in skeletal muscle remain controversial. Some investigators have demonstrated that heparin can ameliorate ischemic injury to heart, brain, and renal tissue. We investigated the ability of heparin sodium to decrease ischemia-reperfusion injury in an isolated gracilis muscle model in ten anesthetized mongrel dogs. One gracilis muscle was perfused normally while the contralateral muscle was subjected to six hours of ischemia followed by one hour of reperfusion. Five dogs were given a preischemic bolus of heparin sodium (200 U/kg, intravenously followed by a continuous infusion (15 U/kg/h, intravenously), and five control dogs received no heparin. Quantitation of skeletal muscle ischemia-reperfusion injury was determined by histochemical staining with triphenyl tetrazolium-chloride and computerized planimetry of the infarct size. Results from the ischemic muscle demonstrate a significant beneficial effect of heparinization. The nonheparinized dogs had a 72% +/- 5% infarct size, which was significantly reduced to 24% +/- 8% in the heparinized dogs. The mechanism of this protective effect may be due to heparin's anticoagulant, antiplatelet, or anti-inflammatory action.