Zinc-modified titanium surface enhances osteoblast differentiation of dental pulp stem cells in vitro.
Zinc-modified titanium surface enhances osteoblast differentiation of dental pulp stem cells in vitro.
复制标题
锌改性的钛表面在体外增强了牙髓干细胞的成骨细胞分化。
DOI：
10.1038/srep29462
复制
发表时间：
2016-07-08
影响因子：
4.6
通讯作者：
Iino M
中科院分区：
文献类型：
--
作者：
Yusa K;Yamamoto O;Takano H;Fukuda M;Iino M
锌是一种必需的微量元素，在成骨细胞分化和骨建模中发挥重要作用。这项体外研究旨在评估人牙髓干细胞 (DPSC) 在锌修饰钛 (Zn-Ti) 上的成骨细胞分化，锌修饰钛 (Zn-Ti) 从其表面释放锌离子。基于实时 PCR、碱性磷酸酶 (ALP) 活性和蛋白质印迹分析数据，我们研究了在 Zn-Ti 和对照上培养的 DPSC 的成骨细胞分化。与对照组相比，在 Zn-Ti 上培养的 DPSC 的 I 型胶原 (Col I)、骨形态发生蛋白 2 (BMP2)、ALP、runt 相关转录因子 2 (Runx2)、骨桥蛋白 (OPN) 和血管内皮生长因子 A (VEGF A) 等成骨细胞相关基因的基因表达水平显着上调。我们还通过茜素红 S (ARS) 染色研究了细胞外基质 (ECM) 矿化，发现与对照相比，Zn-Ti 显着促进了 ECM 矿化。这些发现表明，Zn-Ti 和 DPSC 的组合为骨再生治疗提供了一种新方法。
Zinc is an essential trace element that plays an important role in differentiation of osteoblasts and bone modeling. This in vitro study aimed to evaluate the osteoblast differentiation of human dental pulp stem cells (DPSCs) on zinc-modified titanium (Zn-Ti) that releases zinc ions from its surface. Based on real-time PCR, alkaline phosphatase (ALP) activity and Western blot analysis data, we investigated osteoblast differentiation of DPSCs cultured on Zn-Ti and controls. DPSCs cultured on Zn-Ti exhibited significantly up-regulated gene expression levels of osteoblast-related genes of type I collagen (Col I), bone morphogenetic protein 2 (BMP2), ALP, runt-related transcription factor 2 (Runx2), osteopontin (OPN), and vascular endothelial growth factor A (VEGF A), as compared with controls. We also investigated extracellular matrix (ECM) mineralization by Alizarin Red S (ARS) staining and found that Zn-Ti significantly promoted ECM mineralization when compared with controls. These findings suggest that the combination of Zn-Ti and DPSCs provides a novel approach for bone regeneration therapy.