Hybridisation between turtle subspecies: a case study with the European pond turtle (Emys orbicularis)
Hybridisation between turtle subspecies: a case study with the European pond turtle (Emys orbicularis)
复制标题
DOI：
10.1007/s10592-016-0901-y
复制
发表时间：
2017-04
影响因子：
2.2
通讯作者：
Matthieu Raemy;U. Fritz;M. Cheylan;S. Ursenbacher
中科院分区：
文献类型：
--
作者：
Matthieu Raemy;U. Fritz;M. Cheylan;S. Ursenbacher
基因渗入最近已成为保护遗传学的一个重要关注点，因为它可能发生在稀有物种和相关常见物种之间、不同遗传群体之间以及来自不同起源的个体之间。我们的目标是：（i）确定法国自然接触区的圆环艾米龟亚种之间是否发生杂交或基因渗入； (ii) 确定法国不同亚种之间接触区的地理范围； (iii) 评估渗入发生在哪个水平，最后； (iv)评估线粒体和核遗传标记的组合是否反映了与仅使用一种遗传标记时相同的接触区。通过对法国人群的双亲遗传微卫星进行基因分型并对母系遗传的细胞色素b基因进行测序来评估基因渗入。我们在法国南部和东部的旧接触带的自然条件下证明了亚种之间的强烈渗入。我们的结果证实基因渗入反映了过去的自然事件，但也证明了人类的影响改变了这些模式。我们最终证实，线粒体和核遗传标记的组合比仅使用一种遗传标记更适合揭示基因渗入。
Genetic introgression has recently become an important concern for conservation genetics as it can occur between rare and related common species, between various genetic groups and between individuals from different origins. Our aims were: (i) to determine whether hybridisation or introgression occurs betweenEmys orbicularissubspecies in a natural contact zone in France; (ii) to determine the geographic extent of the contact zone between distinct subspecies in France; (iii) to evaluate at which level introgression occurs, and finally; (iv) to evaluate whether combining mitochondrial and nuclear genetic markers reflects the same contact zone as when only one genetic marker is used. Introgression was evaluated by genotyping biparentally inherited microsatellites and sequencing the maternally inherited cytochromebgene of French populations. We demonstrated strong introgression between subspecies under natural conditions in the old contact zone in southern and eastern France. Our results corroborated that introgression reflects past natural events, but also demonstrated that human impact has altered these patterns. We finally confirmed that the combination of mitochondrial and nuclear genetic markers is more appropriate to reveal introgression than the use of only one genetic marker.