Rapid customization of Solanaceae fruit crops for urban agriculture
Rapid customization of Solanaceae fruit crops for urban agriculture
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DOI：
10.1038/s41587-019-0361-2
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发表时间：
2019-12-23
影响因子：
46.9
通讯作者：
Lippman, Zachary B.
中科院分区：
文献类型：
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作者：
Kwon, Choon-Tak;Heo, Jung;Lippman, Zachary B.
在城市环境中种植农作物可能会减少粮食生产对环境的影响（1-4）。然而，城市中可用土地的缺乏和快速作物循环的需要，以快速和连续地产生，意味着到目前为止，只有生菜和相关的“绿叶绿色”蔬菜在城市农场中种植（5）。具有适合城市农业的结构和产量的新水果品种已被证明很难繁殖（1，5）。我们确定了番茄茎长（SLER）的调节因子，并设计了一种性状叠加策略，以结合联合收割机突变来获得浓缩枝、快速开花（SP 5G）和早熟生长终止（SP）。我们使用一步CRISPR-Cas9基因组编辑的策略的应用将藤状番茄植物重组为适合城市农业的紧凑，早期产量的植物。田间数据证实，产量得到了保持，我们证明了室内耕作系统的种植。在另一种茄科植物地樱桃中单独靶向相同的茎长调节剂，也使得能够工程化到紧凑的身材。我们的方法可以扩大城市农业的作物品种库。使用基因组编辑生产适合城市农业的紧凑早实番茄和地樱桃植物。
Cultivation of crops in urban environments might reduce the environmental impact of food production(1-4). However, lack of available land in cities and a need for rapid crop cycling, to yield quickly and continuously, mean that so far only lettuce and related 'leafy green' vegetables are cultivated in urban farms(5). New fruit varieties with architectures and yields suitable for urban farming have proven difficult to breed(1,5). We identified a regulator of tomato stem length (SlER) and devised a trait-stacking strategy to combine mutations for condensed shoots, rapid flowering (SP5G) and precocious growth termination (SP). Application of our strategy using one-step CRISPR-Cas9 genome editing restructured vine-like tomato plants into compact, early yielding plants suitable for urban agriculture. Field data confirmed that yields were maintained, and we demonstrated cultivation in indoor farming systems. Targeting the same stem length regulator alone in groundcherry, another Solanaceae plant, also enabled engineering to a compact stature. Our approach can expand the repertoire of crops for urban agriculture.Compact early fruiting tomato and groundcherry plants suitable for urban farming are produced using genome editing.