Reciprocal control of the conformational state of the sarcoplasmic reticulum calcium channel protein by polarization and depolarization in the transverse tubule.
Reciprocal control of the conformational state of the sarcoplasmic reticulum calcium channel protein by polarization and depolarization in the transverse tubule.
复制标题
通过横管中的极化和去极化相互控制肌浆网钙通道蛋白的构象状态。
DOI：
10.1016/0014-5793(96)00973-8
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发表时间：
1996
期刊：
影响因子：
3.5
通讯作者：
El-Hayek,R
中科院分区：
文献类型：
--
作者：
Ikemoto,N;El-Hayek,R
我们将构象探针甲基香豆素醋酸酯（MCA）特异地连接到三联体的连接足蛋白（JFP）片段上，并监测了在T小管片段极化和去极化过程中JFP的构象变化。当T小管极化时，MCA荧光减弱，通过阻止T小管极化或尼莫地平阻断T小管与肌浆网的通讯，可以阻断荧光变化。T管的去极化逆转了由T管极化引起的MCA荧光减弱。这些结果表明，JFP的构象和功能状态是由T管极化和退极化以一种互反的方式调节的。
We attached the conformational probe methylcoumarin acetate (MCA) specifically to the junctional foot protein (JFP) moiety of triads, and monitored conformational changes in the JFP during polarization and depolarization of the T‐tubule moiety. The MCA fluorescence decreased upon T‐tubule polarization, and the fluorescence changes were blocked by preventing T‐tubule polarization or by a nimodipine block of the T‐tubule‐to‐sarcoplasmic reticulum communication. Depolarization of the T‐tubule reversed the MCA fluorescence decrease which had been produced by T‐tubule polarization. These results suggest that the conformational and functional states of the JFP are regulated by T‐tubule polarization and depolarization in a reciprocal fashion.