Deep Tissue Microscopic Imaging of the Kidney with a Gradient-Index Lens System.
Deep Tissue Microscopic Imaging of the Kidney with a Gradient-Index Lens System.
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DOI：
10.1016/j.optcom.2007.08.074
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发表时间：
2008-04
影响因子：
2.4
通讯作者：
Xin Li;Weiming Yu
中科院分区：
文献类型：
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作者：
Xin Li;Weiming Yu
使用双光子激发的活体显微镜被证明是研究肾脏和相关疾病过程的有价值的工具。然而，肾脏活体成像的常规性能受到荧光信号被组织衰减的限制，并且在某些组织深度完全失去其强度。对于大多数动物组织，这种有限的成像深度被限制在几百微米。目前，在皮质的浅表组织层之外对肾脏进行无创成像是不可能的。这对用于成像的动物模型施加了显著的限制，因为肾小球等结构通常位于皮质浅层以下，不能使用传统的荧光显微镜成像。在这里，我们报告使用基于梯度指数（GRIN）微透镜的针状透镜系统，能够通过常规显微镜物镜传输高质量的组织荧光图像，用于肾脏深层组织成像。通过将GRIN透镜系统与蔡司LSM 510 NLOZ显微镜相结合，我们能够将肾脏组织的成像深度远远超出几百微米的限制。这种GRIN透镜成像系统提供了一种替代的显微内窥镜成像工具，将增强当前的活体肾脏成像技术，用于研究肾脏表层以下局部组织的结构和功能特性。
Intravital microscopy using two-photon excitation is proven to be a valuable tool for studying the kidney and associated disease processes. However, routine performance of intravital kidney imaging is limited by the fact that fluorescence signal is attenuated by the tissue and at certain tissue depth lost its strength completely. For most of the animal tissues, this finite imaging depth is limited to a few hundred microns. Currently it is not possible to non-invasively image the kidney beyond the superficial tissue layers of the cortex. This has imposed significant limitations on the animal models one can use for imaging since structure such the glomerulus is typically located below the superficial layer of the cortex that cannot be imaged using a conventional fluorescence microscope. Here we report the use of a needle-like lens system based on gradient-index (GRIN) microlenses capable of transferring high quality fluorescence images of the tissue through a regular microscope objective for deep tissue imaging of the kidney. By combining this GRIN lens system with a Zeiss LSM 510 NLOZ microscope, we are able to extend the imaging depth for kidney tissues far beyond the few hundred microns limit. This GRIN lens imaging system provides an alternative microendoscopic imaging tool that will enhance current intravital kidney imaging techniques for studying structural and functional properties of local tissues at locations below the superficial layers of the kidney.