Confocal laser scanning microscopic observation of glycocalyx production by Staphylococcus aureus in skin lesions of bullous impetigo, atopic dermatitis and pemphigus foliaceus
Confocal laser scanning microscopic observation of glycocalyx production by Staphylococcus aureus in skin lesions of bullous impetigo, atopic dermatitis and pemphigus foliaceus
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DOI：
10.1046/j.1365-2133.2003.05162.x
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发表时间：
2003-03-01
影响因子：
10.3
通讯作者：
Iwatsuki, K
中科院分区：
文献类型：
--
作者：
Akiyama, H;Hamada, T;Iwatsuki, K
糖萼在脱水过程中崩塌，产生电子密集的吸积。共聚焦激光扫描显微镜（CLSM）可用于观察完全水合的微生物生物膜。目的应用CLSM分析金黄色葡萄球菌在大疱性脓疱病、特应性皮炎和天疱疮皮损中的糖萼生成。第二个目的是比较常规光学显微镜下不同方法制备的组织切片中金黄色葡萄球菌细胞的数量。方法对脓疱疮、特应性皮炎和天疱疮皮损中的金黄色葡萄球菌细胞进行红花红染色，异硫氰酸偶联灯头蛋白A荧光染色阳性，认为存在糖萼。结果脓疱疮、特应性皮炎和天疱疮皮损的金黄色葡萄球菌细胞均被糖萼覆盖并形成小菌落。常规光镜切片中的金黄色葡萄球菌细胞数量明显低于未用乙醇脱水的冷冻切片。结论金黄色葡萄球菌在大疱性脓疱、特应性皮炎和天疱疮皮损中普遍产生糖萼，这是金黄色葡萄球菌难以从这些皮损中去除的原因。糖萼可能在脱水过程中塌陷，在常规光镜切片的制备过程中，大多数金黄色葡萄球菌细胞可能被带走。
Background Glycocalyx collapses during dehydration to produce electron-dense accretions. Confocal laser scanning microscopy (CLSM) may be used to visualize fully hydrated microbial biofilms.Objectives Using CLSM, to analyse glycocalyx production by Staphylococcus aureus cells in skin lesions of bullous impetigo, atopic dermatitis and pemphigus foliaceus. A second objective was to compare numbers of S. aureus cells in tissue sections prepared by different methods for routine light microscopy.Methods S. aureus cells in skin lesions of impetigo, atopic dermatitis and pemphigus were stained with safranin, and positive staining with fluorescein isothiocyanate-conjugated concanavalin A was considered to indicate the presence of glycocalyx.Results All S. aureus cells tested in skin lesions of impetigo, atopic dermatitis and pemphigus were covered with glycocalyx and formed microcolonies. The numbers of S. aureus cells in a routine light microscopy section were significantly lower than those in a frozen section that had not been dehydrated with ethanol.Conclusions S. aureus cells generally produce glycocalyx in skin lesions of bullous impetigo, atopic dermatitis and pemphigus foliaceus, which accounts for the difficulty of removing S. aureus cells from these skin lesions. The glycocalyx may collapse during dehydration and most of the S. aureus cells may be carried away during preparation of routine light microscope sections.