NMDA Receptor-Dependent Multidendrite Ca2+ Spikes Required for Hippocampal Burst Firing In Vivo
NMDA Receptor-Dependent Multidendrite Ca2+ Spikes Required for Hippocampal Burst Firing In Vivo
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DOI：
10.1016/j.neuron.2014.01.014
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发表时间：
2014-03-19
期刊：
影响因子：
16.2
通讯作者：
Konnerth, Arthur
中科院分区：
文献类型：
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作者：
Grienberger, Christine;Chen, Xiaowei;Konnerth, Arthur
动作电位（AP）的高频爆发是哺乳动物中枢神经元中一种独特的信号形式。在体内海马CA 1区锥体神经元中，在各种行为中检测到这种复杂的尖峰脉冲（CS），并且被认为对学习和记忆相关的突触可塑性特别重要。在这里，我们结合全细胞记录和双光子成像在小鼠CA 1锥体神经元，以探讨在体内CS的细胞机制。我们的研究结果表明，CS是突触起源，因为它们需要N-甲基-D-天冬氨酸（NMDA）受体激活。我们确定电压门控钙通道依赖性，尖峰样去极化的CS的组成部分。这些Ca 2+尖峰总是与广泛传播的大幅度Ca 2+瞬变基底和顶端树突。总之，我们的研究结果揭示了一种类型的NMDA受体依赖性的多树突Ca 2+穗所需的高频爆裂在体内。
High-frequency bursts of action potentials (APs) are a distinctive form of signaling in various types of mammalian central neurons. In CA1 hippocampal pyramidal neurons in vivo, such complex spike bursts (CSs) are detected during various behaviors and are considered to be particularly important for learning-and memory-related synaptic plasticity. Here, we combined whole-cell recordings and two-photon imaging in mouse CA1 pyramidal neurons to investigate the cellular mechanisms underlying CSs in vivo. Our results demonstrate that CSs are of synaptic origin, as they require N-methyl-D-aspartate (NMDA) receptor activation. We identify voltage-gated Ca2+ channel-dependent, spike-like depolarizations as integral components of the CSs. These Ca2+ spikes were invariably associated with wide-spread large-amplitude Ca2+ transients in basal and apical dendrites. Together, our results reveal a type of NMDA receptor-dependent multidendrite Ca2+ spike required for high-frequency bursting in vivo.