The identity of Penicillium sp 1, a major contaminant of the stone chambers in the Takamatsuzuka and Kitora Tumuli in Japan, is Penicillium paneum
The identity of Penicillium sp 1, a major contaminant of the stone chambers in the Takamatsuzuka and Kitora Tumuli in Japan, is Penicillium paneum
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DOI：
10.1007/s10482-009-9373-0
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发表时间：
2009-11-01
影响因子：
2.6
通讯作者：
Sugiyama, Junta
中科院分区：
文献类型：
--
作者：
An, Kwang-Deuk;Kiyuna, Tomohiko;Sugiyama, Junta
青霉菌是位于奈良县Asuka村的Takamatsuzuka Tumulus （TT）和Kitora Tumulus （KT）石室中的主要居民，这与1300年前的壁画、石膏墙和天花板的生物退化有关。在TT（2004年5月-2007年5月）和KT（2004年6月-2007年9月）373份样品中分离的662株青霉菌中，181株表型为青霉菌1，与青霉菌亚群中Roqueforti节的表型特征相似。青霉菌。选取具有代表性的15株青霉菌进行分子系统发育分析，其中13株来自TT， 2株来自KT。基于28S rDNA D1/D2、ITS、β -微管蛋白和lys2基因序列的系统发育分析清楚地表明，已知的三种罗克福氏蜱、P. carneum和P. paneum与所有TT和KT分离株属于同一组。除此之外，TT和KT分离株与P. paneum Frisvad的前全型菌株CBS 101032形成了一个单系群，具有很强的bootstrap支持。到目前为止，大肠杆菌只从发霉的黑麦面包、其他食物和捆草青贮饲料中分离出来。因此，这是第一次从石膏墙上的壁画等生物变质文物样品中分离出paneum。
Penicillium appeared as the major dweller in the Takamatsuzuka Tumulus (TT) and Kitora Tumulus (KT) stone chambers, both located in the village of Asuka, Nara Prefecture, in relation to the biodeterioration of the 1,300-year-old mural paintings, plaster walls and ceilings. Of 662 Penicillium isolates from 373 samples of the TT (sampling period, May 2004-2007) and the KT (sampling period, June 2004-Sep 2007), 181 were phenotypically assigned as Penicillium sp. 1 which shared similar phenotypic characteristics of sect. Roqueforti in Penicillium subg. Penicillium. Fifteen representative isolates of Penicillium sp. 1, 13 from TT and 2 from KT, were selected for molecular phylogenetic analysis. The 28S rDNA D1/D2, ITS, beta-tubulin, and lys2 gene sequence-based phylogenies clearly demonstrated that the three known species P. roqueforti, P. carneum and P. paneum in sect. Roqueforti, and all TT and KT isolates grouped together. In addition to this, TT and KT isolates formed a monophyletic group with the ex-holotype strain CBS 101032 of P. paneum Frisvad with very strong bootstrap supports. So far, P. paneum has been isolated only from mouldy rye breads, other foods, and baled grass silage. Therefore, this is the first report of P. paneum isolation from samples relating to the biodeteriorated cultural properties such as mural paintings on plaster walls.