Inhibitory mechanism of saponins derived from roots of Platycodon grandiflorum on anaphylactic reaction and IgE-mediated allergic response in mast cells
Inhibitory mechanism of saponins derived from roots of Platycodon grandiflorum on anaphylactic reaction and IgE-mediated allergic response in mast cells
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DOI：
10.1016/j.fct.2009.01.041
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发表时间：
2009-06-01
影响因子：
4.3
通讯作者：
Jeong, Hye Gwang
中科院分区：
文献类型：
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作者：
Han, Eun Hee;Park, Lin Hee;Jeong, Hye Gwang
本研究旨在探讨桔梗皂苷（Changeplasticsaponins：CKS）对小鼠过敏模型及肥大细胞的保护作用。口服CKS可抑制DNP-IgE抗体诱导的小鼠全身PCA反应。CKS减少抗DNP-IgE致敏的RBL-2 H3细胞的β-氨基己糖苷酶和组胺释放。此外，CKS抑制IgE抗体诱导的RBL-2 H3细胞中IL-4和TNF-α产生和表达的增加。为了探讨CKS抑制PCA和肥大细胞脱颗粒的机制，我们检测了细胞内信号分子的激活。CKS抑制DNP-IgE抗体诱导的Syk磷酸化。在下游，CKS还抑制Akt和MAP激酶的磷酸化。总而言之，CKS的体内/体外抗过敏作用表明该药物可能通过抑制炎症细胞因子和Syk依赖性信号级联反应来治疗过敏性疾病。(C)2009爱思唯尔有限公司保留所有权利。
The purpose of this study was to investigate the protective effects of saponins isolated from the root of Platycodi Radix (Changkil saponins: CKS) anti-allergic effects in mice and mast cells. Oral administration of CKS inhibited the dinitrophenyl (DNP)-IgE antibody-induced systemic PCA reaction in mice. CKS reduced the beta-hexosaminidase and histamine release from anti-DNP-IgE-sensitized RBL-2H3 cells. In addition, CKS inhibited the IgE antibody-induced increases in IL-4 and TNF-alpha production and expression in RBL-2H3 cells. In order to explore the inhibitory mechanism of CKS in PCA and mast cell degranulation, we examined the activation of intracellular signaling molecules. CKS suppressed DNP-IgE antibody-induced Syk phosphorylation. Further downstream, CKS also inhibited the phosphorylation of Akt and MAP kinases. Taken together, the in vivo/in vitro anti-allergic effects of CKS suggest possible therapeutic applications for this agent in allergic diseases through the inhibition of inflammatory cytokines and Syk-dependent signaling cascades. (C) 2009 Elsevier Ltd. All rights reserved.