Soil Fungal Communities Respond to Grassland Plant Community Richness and Soil Edaphics
Soil Fungal Communities Respond to Grassland Plant Community Richness and Soil Edaphics
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DOI：
10.1007/s00248-014-0531-1
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发表时间：
2015-07-01
影响因子：
3.6
通讯作者：
Kistler, H. Corby
中科院分区：
文献类型：
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作者：
LeBlanc, Nicholas;Kinkel, Linda L.;Kistler, H. Corby
土壤真菌群落对陆地生态系统动态具有重要影响，但目前对土壤真菌多样性和群落结构的驱动因素的认识还很有限。真菌群落与四个本地多年生草原植物物种，两个豆科植物和两个草，在长期的田间试验中生长在单一栽培和混养的根际的特点。参考数据库的开发，扩增子库测序，多拷贝rRNA和单拷贝蛋白质编码基因座。利用聚类和基于聚类的管道来评估真菌群落结构和多样性的差异，以响应植物宿主，植物群落丰富度和土壤土壤蚜虫。与单作植物群落相比，混作植物群落中植物根际真菌多样性增加。真菌群落结构之间的豆科植物和禾本科植物生长在单一的文化，但不是在混养分化。为了更好地监测土壤中镰刀菌属真菌的分布，本研究利用镰刀菌蛋白编码基因提高了系统发育分辨率，并对镰刀菌进行了富集，结果表明，土壤中镰刀菌群落的丰富度和结构与植物群落动态和土壤特性密切相关。
Fungal communities in soil have significant influences on terrestrial ecosystem dynamics, yet our understanding of the drivers of fungal diversity and community structure in soil is limited. Fungal communities associated with the rhizosphere of four native perennial grassland plant species, two legumes and two grasses, grown in monoculture and polyculture in a long-term field experiment were characterized. Reference databases were developed for, and amplicon libraries sequenced from, multiple-copy rRNA and single-copy protein-coding loci. Clustering and alignment-based pipelines were utilized to evaluate differences in fungal community structure and diversity in response to plant host, plant community richness, and soil edaphics. Fungal diversity increased in the rhizosphere of plants growing in polyculture plant communities as compared to monoculture plant communities. Fungal community structure was differentiated between legumes and grasses growing in monoculture but not in polyculture. To specifically monitor fungi in the genus Fusarium in the soil, the protein-coding locus was used to increase phylogenetic resolution and enrich for this taxon. These data show that fungal community richness and structure are strongly linked with plant community dynamics and associated soil edaphic characteristics in these grassland soils.