Genome-Wide Study of Pseudomonas aeruginosa Outer Membrane Protein Immunogenicity Using Self-Assembling Protein Microarrays
Genome-Wide Study of Pseudomonas aeruginosa Outer Membrane Protein Immunogenicity Using Self-Assembling Protein Microarrays
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DOI：
10.1128/iai.00698-09
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发表时间：
2009-11-01
影响因子：
3.1
通讯作者：
LaBaer, Joshua
中科院分区：
文献类型：
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作者：
Montor, Wagner R.;Huang, Jin;LaBaer, Joshua
铜绿假单胞菌对防御机制受损的个体和囊性纤维化患者的潜在威胁生命的感染负责。铜绿假单胞菌感染表现为对某些已知抗原（如鞭毛蛋白C、弹性蛋白酶、碱性蛋白酶等）产生体液反应。虽然已知许多免疫原性蛋白质，但尚未批准有效的疫苗。在这里，我们报告了一个全面的研究所有262外膜和出口铜绿假单胞菌PAO1蛋白通过一个改进的蛋白质微阵列方法称为核酸可编程蛋白阵列。从这项研究中，有可能鉴定出在囊性纤维化和急性感染患者中触发适应性免疫反应的12种蛋白质，为在感染的自然过程中体内免疫系统实际识别哪些细菌蛋白质提供了有价值的信息。这些蛋白在患者和对照组的差异检测证明有统计学意义（P < 0.01）。该研究为改善血清学诊断和开发疫苗提供了一份潜在候选名单。
Pseudomonas aeruginosa is responsible for potentially life-threatening infections in individuals with compromised defense mechanisms and those with cystic fibrosis. P. aeruginosa infection is notable for the appearance of a humoral response to some known antigens, such as flagellin C, elastase, alkaline protease, and others. Although a number of immunogenic proteins are known, no effective vaccine has been approved yet. Here, we report a comprehensive study of all 262 outer membrane and exported P. aeruginosa PAO1 proteins by a modified protein microarray methodology called the nucleic acid-programmable protein array. From this study, it was possible to identify 12 proteins that trigger an adaptive immune response in cystic fibrosis and acutely infected patients, providing valuable information about which bacterial proteins are actually recognized by the immune system in vivo during the natural course of infection. The differential detections of these proteins in patients and controls proved to be statistically significant (P < 0.01). The study provides a list of potential candidates for the improvement of serological diagnostics and the development of vaccines.