IMMUNOCYTOCHEMICAL LOCALIZATION OF COLLAGENS (IV) IN THE HUMAN IRIS

IMMUNOCYTOCHEMICAL LOCALIZATION OF COLLAGENS (IV) IN THE HUMAN IRIS
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DOI:
10.1007/bf02764315
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发表时间:
1990-01-01
影响因子:
2.7
通讯作者:
LEE, WR
LEE, WR
中科院分区:
医学3区
文献类型:
--
作者:
KONSTAS, AG;MARSHALL, GE;LEE, WR

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在本研究中,我们采用低温显微切开术和伦敦树脂白塑料包埋技术在精细结构水平上研究了I-V型胶原蛋白在人虹膜中的分布。I型胶原存在于虹膜血管基底膜中,而III型胶原则不存在;I型和III型均存在于虹膜基质中。IV型胶原是血管细胞、肌上皮细胞、成纤维细胞和上皮细胞基底膜的主要成分。II型和V型未见。低温和塑料埋入技术都产生了非常相似的结果。
In this study we investigated the distribution of collagen types I-V in the human iris at the fine-structural level using cryoultramicrotomy and London Resin White plastic embedding. Collagen type I was shown to be present in the basement membrane of iris vessels, in contrast to type III, which was absent; both types I and III were present in the iris stroma. Collagen type IV was a major component of basement membranes of vascular cells, myoepithelial cells, fibroblasts and epithelial cells. Types II and V were absent. Both cryo and plastic embedding techniques produced closely comparable results.