Silk sericin protein of tropical tasar silkworm inhibits UVB-induced apoptosis in human skin keratinocytes
Silk sericin protein of tropical tasar silkworm inhibits UVB-induced apoptosis in human skin keratinocytes
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DOI：
10.1007/s11010-008-9702-z
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发表时间：
2008-04-01
影响因子：
4.3
通讯作者：
Kundu, S. C.
中科院分区：
文献类型：
--
作者：
Dash, Rupesh;Mandal, Mahitosh;Kundu, S. C.
在小鼠皮肤模型中，蚕丝蛋白中的丝胶蛋白被证实是一种有效的抗氧化剂和抗紫外线B（UVB）辐射的光保护剂。在这项研究中，我们研究了在UVB（30 mJ/cm（2））照射的人角质形成细胞中，丝胶蛋白的抗凋亡作用。流式细胞仪分析表明，用丝胶蛋白预处理抑制UVB诱导的细胞凋亡。丝胶预处理抑制bax表达，上调bcl-2表达，抑制caspase-3的激活和Poly（ADP-核糖）聚合酶的裂解。在UVB处理角质形成细胞的细胞内过氧化氢的产生被抑制，通过预处理与丝胶蛋白，表明丝胶蛋白可能防止线粒体损伤。
The silk protein sericin has been identified as a potent antioxidant and photoprotective agent against ultraviolet B (UVB) irradiation in mouse skin model. In this study, we have investigated the anti-apoptotic effect of sericin in UVB (30 mJ/cm(2))-irradiated human keratinocytes. Flow cytometry analysis has shown that pre-treatment with sericin inhibits UVB-induced apoptosis. The pre-treatment with sericin suppresses bax expression, up-regulates the expression of bcl-2, prevents both the activation of caspase-3 and cleavage of Poly (ADP-ribose) polymerase. Generation of intracellular hydrogen peroxide in UVB-treated keratinocytes is inhibited through pre-treatment with sericin suggesting that sericin probably prevents mitochondrial damage.