Parvalbumin Interneurons of Hippocampus Tune Population Activity at Theta Frequency
Parvalbumin Interneurons of Hippocampus Tune Population Activity at Theta Frequency
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DOI：
10.1016/j.neuron.2015.05.027
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发表时间：
2015-06-03
期刊：
影响因子：
16.2
通讯作者：
Williams, Sylvain
中科院分区：
文献类型：
--
作者：
Amilhon, Benedicte;Huh, Carey Y. L.;Williams, Sylvain
海马theta节律源于最近描述的内在theta振荡器和来自多个大脑区域的输入的组合。中间神经元表达的标记物小清蛋白（PV）和生长抑素（SOM）的主要候选人参与的内在节奏的产生和主细胞（PC）的协调在远端CA1和下托。我们测试了他们的参与，通过光遗传学激活和沉默PV或SOM中间神经元在一个完整的海马制备，保留内在的连接和振荡自发地在θ频率。尽管有证据表明SOM中间神经元对θ至关重要，但这些中间神经元的光遗传学操作适度影响θ节律。然而，SOM中间神经元能够强烈调节临时氨输入。相比之下，PV中间神经元的激活在8 Hz时有力地控制了PC网络和节律的产生，而持续沉默它们则破坏了θ。因此，我们的研究结果表明，PV，但不是SOM中间神经元PC同步和出现的内在海马θ的关键作用。
Hippocampal theta rhythm arises from a combination of recently described intrinsic theta oscillators and inputs from multiple brain areas. Interneurons expressing the markers parvalbumin (PV) and somatostatin (SOM) are leading candidates to participate in intrinsic rhythm generation and principal cell (PC) coordination in distal CA1 and subiculum. We tested their involvement by optogenetically activating and silencing PV or SOM interneurons in an intact hippocampus preparation that preserves intrinsic connections and oscillates spontaneously at theta frequencies. Despite evidence suggesting that SOM interneurons are crucial for theta, optogenetic manipulation of these interneurons modestly influenced theta rhythm. However, SOM interneurons were able to strongly modulate temporoammonic inputs. In contrast, activation of PV interneurons powerfully controlled PC network and rhythm generation optimally at 8 Hz, while continuously silencing them disrupted theta. Our results thus demonstrate a pivotal role of PV but not SOM interneurons for PC synchronization and the emergence of intrinsic hippocampal theta.