HETEROGENEITY OF THE HYPOXIC STATE IN RAT-HEART IS DETERMINED AT CAPILLARY LEVEL
HETEROGENEITY OF THE HYPOXIC STATE IN RAT-HEART IS DETERMINED AT CAPILLARY LEVEL
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DOI：
10.1152/ajpheart.1993.264.2.h294
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发表时间：
1993-02-01
影响因子：
--
通讯作者：
BRUINING, HA
中科院分区：
文献类型：
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作者：
INCE, C;ASHRUF, JF;BRUINING, HA
本文用还原型烟酰胺腺嘌呤二核苷酸（NADH）和钯卟啉荧光法研究了缺氧状态下酪氨酸灌注大鼠心脏的异源性。缺血以及高流量缺氧导致组织NADH荧光的均匀上升，而从这两种类型的缺氧恢复常氧引起短暂持续斑片状荧光区。每颗心脏的模式都是一样的。通过Pd-卟啉磷光测量的血管中的PO 2分布显示了与NADH荧光模式相似的模式。微球栓塞的毛细血管，但不是小动脉，引起相同的NADH荧光模式，从缺氧恢复过程中看到没有微球。高心率也会引起片状荧光区域，但在腺苷存在的情况下不会。模式对应于在低心率下从缺氧恢复到常氧时所观察到的模式。得出结论，有循环单位在大鼠心脏的毛细血管水平，导致暂时持续的缺氧地区在恢复缺氧。这些脆弱的区域是在高心跳率期间首先受到损害的。
Heterogeneity in the hypoxic state of Tyrode-perfused rat hearts was studied using NADH and Pd-porphine videofluorometry. Ischemic as well as high-flow anoxia resulted in a homogeneous rise of tissue NADH fluorescence, whereas normoxic recovery from both types of anoxia caused transiently persisting patchy fluorescent areas. Patterns were always the same for a given heart. PO2 distribution in the vasculature measured by Pd-porphine phosphorescence showed patterns similar to the NADH fluorescence patterns. Microsphere embolization of the capillaries, but not of arterioles, elicited identical NADH fluorescence patterns as seen during recovery from anoxia without microspheres. High heartbeat rates also caused patchy fluorescent areas but not in the presence of adenosine. Patterns corresponded to those seen during normoxic recovery from anoxia under low beat rates. It is concluded that there are circulatory units in the rat heart at the capillary level that result in the temporary persistence of anoxic areas during recovery from anoxia. These vulnerable areas are the first to be compromised during high heartbeat rates.