KymoAnalyzer: a software tool for the quantitative analysis of intracellular transport in neurons.
KymoAnalyzer: a software tool for the quantitative analysis of intracellular transport in neurons.
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DOI：
10.1111/tra.12456
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发表时间：
2017-01
期刊：
影响因子：
--
通讯作者：
Encalada SE
中科院分区：
文献类型：
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作者：
Neumann S;Chassefeyre R;Campbell GE;Encalada SE
在轴突中，蛋白质、囊泡、细胞器和其他货物的适当定位是通过高度调控的动蛋白和动力蛋白的协调完成的，动力蛋白是与货物结合并沿微管(MT)轨迹转移的分子马达。轴突运输障碍与多种神经退行性疾病的发病机制有关，包括阿尔茨海默病和亨廷顿病。为了了解基于MT的货物运动是如何被调节的，并描述其在神经退行性变中的作用，分析轴突内货物移动的详细动力学是至关重要的。在这里，我们介绍KymoAnalyzer，这是一个软件工具，有助于从延时实时成像序列中稳健地分析轴突运输。KymoAnalyzer是一款开源软件，它自动对粒子轨迹进行分类，并系统地计算速度、运行长度、停顿和大量其他参数，这些都是基于电机的运输的特点。我们预计，实验室将很容易地使用这一包来揭示以前发现的神经元内单独移动货物的细胞内运输细节。
In axons, proper localization of proteins, vesicles, organelles, and other cargoes is accomplished by the highly regulated coordination of kinesins and dyneins, molecular motors that bind to cargoes and translocate them along microtubule (MT) tracks. Impairment of axonal transport is implicated in the pathogenesis of multiple neurodegenerative disorders including Alzheimer’s and Huntington’s diseases. To understand how MT-based cargo motility is regulated and to delineate its role in neurodegeneration, it is critical to analyze the detailed dynamics of moving cargoes inside axons. Here, we present KymoAnalyzer, a software tool that facilitates the robust analysis of axonal transport from time-lapse live-imaging sequences. KymoAnalyzer is an open-source software that automatically classifies particle trajectories and systematically calculates velocities, run lengths, pauses, and a wealth of other parameters that are characteristic of motor-based transport. We anticipate that laboratories will easily use this package to unveil previously uncovered intracellular transport details of individually-moving cargoes inside neurons.