An integrated-molecule-format multicolor probe for monitoring multiple activities of a bioactive small molecule
An integrated-molecule-format multicolor probe for monitoring multiple activities of a bioactive small molecule
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DOI：
10.1021/cb800004s
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发表时间：
2008-06-01
影响因子：
4
通讯作者：
Tao, Hiroaki
中科院分区：
文献类型：
--
作者：
Kim, Sung Bae;Umezawa, Yoshio;Tao, Hiroaki
Bioactive small molecules, including steroids, activate multiple signaling pathways in mammalian cells. However, current technologies cannot illuminate such multiple effects of a ligand in mammalian cells. Here, we demonstrate integrated-molecule-format multicolor systems simultaneously visualizing bifacial activities of a ligand, where estrogen receptor alpha (ER alpha) was exemplified to demonstrate the present technology. First, we developed a single-molecule-format probe emitting red bioluminescence for imaging interaction between the phosphorylated ligand binding domain of ER alpha (ER LBD) and the Src homology-2 (SH2) domain of: Src. The SH2 domain-linked ER LBD was sandwiched between dissected N- and C-terminal fragments of Pyrophorus plagioph thalamus (click beetle) luciferase emitting red bioluminescence. Second, another single-molecule-format bioluminescent probe emitting green bioluminescence was constructed to visualize intramolecular interaction between ER LBD and LXXLL motifs. Mammalian cells carrying the two probes emit red and/or green light in response to agonistic and antagonistic activities of a ligand, which correspond to its genomic and nongenomic activities, respectively. Third, the two probes were assembled to make an single-molecule-format multicolor indicator, in which all of the components for ligand sensing and multiple-light emission were integrated. The probe emitted characteristic light spectra in response to various agonists and antagonists. This is the first example where (i) protein phosphorylation was recognized with a single bioluminescent probe and (ii) bifacial activities of a ligand, either agonistic or antagonistic, were simultaneously visualized with multiple colors.
