Transferability of SSR markers among wheat, rye, and triticale
Transferability of SSR markers among wheat, rye, and triticale
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DOI：
10.1007/s00122-003-1532-5
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发表时间：
2004-04-01
影响因子：
5.4
通讯作者：
Dweikat, I
中科院分区：
文献类型：
--
作者：
Kuleung, C;Baenziger, PS;Dweikat, I
简单序列重复(SSR)标记是一种有价值的工具，可用于许多目的，如作图、指纹分析和育种。然而，由于它们的开发成本和劳动强度很高，它们只在一些重要的经济作物中可用。比较图谱显示了密切相关物种之间的高度共线性，这使得它们之间可以交换标记。本研究的目的是研究SSR标记在小麦(Triticum aestivum L.)、黑麦(Secale graale L.)和小黑麦(X Triticosecale Wittmack)之间的转移性。利用148个小麦和28个黑麦微卫星标记对小麦、黑麦和小黑麦各5个品系的基因组DNA进行扩增。小麦SSR标记对黑麦的可转移性为17%，而25%的黑麦标记在小麦中可扩增。在小黑麦中，小麦和黑麦标记的转移率分别为58%和39%。小麦、黑麦和小黑麦的平均多态条带分别为2.6、2.7和2.4条，黑麦的平均多态条带为2.0条，小麦和小黑麦为零。这些可转移的标记现在可以用于这些物种的进一步遗传和育种研究。
Simple sequence repeat (SSR) markers are a valuable tool for many purposes, such as mapping, fingerprinting, and breeding. However, they are only available in some economically important crops because of the high cost and labor intensity involved in their development. Comparative mapping reveals a high degree of colinearity between closely related species, which allows the exchange of markers between them. Our objective was to examine the transferability of SSR markers among wheat (Triticum aestivum L.), rye (Secale cereale L.), and triticale (X Triticosecale Wittmack). One hundred forty-eight wheat and 28 rye SSR markers were used to amplify genomic DNA extracted from five lines each of wheat, rye, and triticale. Transferability of wheat SSR markers to rye was 17%, whereas 25% of rye markers were amplifiable in wheat. In triticale, 58% and 39% transferability was achieved for wheat and rye markers, respectively. Wheat markers gave an average of 2.6, 2.7, and 2.4 polymorphic bands in wheat, rye, and triticale, respectively, while rye markers gave an average of 2.0 in rye and none in wheat and triticale. These transferable markers can now be exploited for further genetic and breeding studies in these species.