Boundaries defined by adhesion molecules during development of the cerebral cortex: the J1/tenascin glycoprotein in the mouse somatosensory cortical barrel field.
Boundaries defined by adhesion molecules during development of the cerebral cortex: the J1/tenascin glycoprotein in the mouse somatosensory cortical barrel field.
复制标题
大脑皮层发育过程中粘附分子定义的边界：小鼠体感皮层桶状区域中的 J1/腱生蛋白糖蛋白。
DOI：
10.1016/s0012-1606(89)80056-9
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发表时间：
1989
影响因子：
2.7
通讯作者：
Schachner,M
中科院分区：
文献类型：
--
作者：
Steindler,DA;Cooper,NG;Faissner,A;Schachner,M
使用单克隆抗体通过免疫细胞化学研究了 200/220 KDa J1 糖蛋白 (J1-200/220) 在小鼠体感皮层发育中触须相关桶状区域内的分布。 JI-200/220 是 L2/HNK-1 粘附分子家族的成员，似乎也是腱蛋白的小鼠同源物。出生后 24 至 48 小时之间，J1/生腱蛋白阳性的桶状结构在体感皮层中可见，该分子存在于预期的桶状边界中。免疫电子显微镜揭示了与神经胶质和神经元质膜以及血管上的神经胶质末端足相关的标记。此时J1/腱生蛋白表达的可能主要来源是星形胶质细胞前体细胞和放射状神经胶质细胞。在假定的星形胶质细胞前体细胞中，在包括高尔基体在内的细胞器内观察到免疫标记。在 P6-7，J1/肌腱蛋白在预期的桶间隔膜中最为普遍。 J1/生腱蛋白阳性桶边界在 P9 上几乎不可见，而在 P16 上未观察到。研究结果表明，Jl/tenascin 代表了我们在使用其他方法进行开发期间看到的先前描述的“隐藏”边界的主要组成部分。在桶状场形成的关键阶段，富含粘附分子的边界的表达表明了此类分子在特定大脑皮层模式形成事件中的作用。
The distribution of the 200/220 KDa J1 glycoprotein (Jl-200/220), within the developing vibrissae-related barrel field of the mouse somatosensory cortex, was studied by immunocytochemistry using a monoclonal antibody. JI-200/220, a member of the L2/HNK-1 family of adhesion molecules, also appears to be the mouse homologue of tenascin. Jl/tenascin-positive barrel-like structures are visible in the somatosensory cortex between 24 and 48 hr after birth, with the molecule present in prospective barrel boundaries. Immunoelectronmicroscopy reveals labeling that is associated with glial and neuronal plasma membranes, as well as glial end-feet on blood vessels. A possible major source of Jl/tenascin expression at this time is astrocyte precursor cells and radial glia. In the putative astrocyte precursor cells, immunolabeling was observed within organelles including the Golgi apparatus. At P6–7 Jl/tenascin is most prevalent within prospective interbarrel septae. Jl/tenascin-positive barrel boundaries are barely visible on P9 and not observed on P16. The findings indicate that Jl/tenascin represents a major component of previously described “hidden” boundaries that we have seen during development using other methodologies. The expression of adhesion molecule-rich boundaries during the critical stages of barrel field formation indicates roles for such molecules during specific cerebral cortical pattern formation events.