Comparative proteome profiling of bleomycin-induced lung toxicity in rats by 2D-nano-LC-MS/MS and spectral counting.
Comparative proteome profiling of bleomycin-induced lung toxicity in rats by 2D-nano-LC-MS/MS and spectral counting.
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DOI：
10.1002/bmc.3857
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发表时间：
2017-04
期刊：
影响因子：
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通讯作者：
Xiang Zhou;Xionghua Sun;Xiaogang Jiang
中科院分区：
文献类型：
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作者：
Xiang Zhou;Xionghua Sun;Xiaogang Jiang
迄今为止，博来霉素诱导的肺毒性的机制尚未完全了解。本研究旨在通过2D-nano-LC-MS/MS和光谱计数比较博莱霉素诱导的肺毒性的蛋白质组谱。通过比较对照组和博来霉素治疗组之间鉴定的蛋白质的光谱计数，我们注意到博来霉素治疗组中有102种蛋白质上调，28种蛋白质下调。在这些差异表达的蛋白质中，选择五种蛋白质通过Western印迹分析进行验证。这五种蛋白质的水平与蛋白质组学结果一致。这些潜在的介质可以促进将博来霉素诱导的肺毒性的潜在机制转化为临床竞技场中的分子靶点。
The mechanisms involved in bleomycin-induced lung toxicity have not been fully understood to date. This work aimed to compare the proteome profiling of bleomycin-induced lung toxicity by using 2D-nano-LC-MS/MS and spectral counting. By comparing the spectral counts of identified proteins between control and bleomycin-treated groups, we noted that 102 proteins were upregulated and 28 proteins were downregulated in the bleomycin-treated group. Among these differently expressed proteins, five proteins were chosen for validation by Western blot analysis. The levels of these five proteins were consistent with proteomic results. These potential mediators can facilitate the translation of the underlying mechanisms of bleomycin-induced lung toxicity to molecular targets in the clinical arena.