Shear Stress Increases the Residence Time of Adhesion of Pseudomonas aeruginosa
Shear Stress Increases the Residence Time of Adhesion of Pseudomonas aeruginosa
复制标题
DOI：
10.1016/j.bpj.2010.11.078
复制
发表时间：
2011-01-19
影响因子：
3.4
通讯作者：
Stone, Howard A.
中科院分区：
文献类型：
--
作者：
Lecuyer, Sigolene;Rusconi, Roberto;Stone, Howard A.
虽然无处不在，但细菌在表面上定居的过程仍然知之甚少。本文报道了壁剪应力对铜绿假单胞菌PA14在玻璃和聚二甲基硅氧烷上早期粘附的影响。表面。我们使用图像分析来测量每个粘附细菌在流动下的停留时间。我们的主要发现是，在两个表面上，细菌的特征停留时间随着剪切应力的增加而近似线性增加（类似于0-3.5 Pa）。为了研究这一现象，我们使用了表面细胞器（I型毛、IV型毛或鞭毛）或细胞外基质产生缺陷的突变菌株。我们的研究结果表明，尽管这些细菌表面特征影响粘附事件的频率和早期脱离的概率，但它们都不负责剪切增强粘附时间的趋势。这些观察结果使我们对细菌在剪切流中的附着机制有了新的认识，并提出了细胞表面其他内在特征的作用，或者是细胞对剪切应力的动态反应。
Although ubiquitous, the processes by which bacteria colonize surfaces remain poorly understood. Here we report results for the influence of the wall shear stress on the early-stage adhesion of Pseudomonas aeruginosa PA14 on glass and polydimethylsiloxane. surfaces. We use image analysis to measure the residence time of each adhering bacterium under flow. Our main finding is that, on either surface, the characteristic residence time of bacteria increases approximately linearly as the shear stress increases (similar to 0-3.5 Pa). To investigate this phenomenon, we used mutant strains defective in surface organelles (type I pili, type IV pili, or the flagellum) or extracellular matrix production. Our results show that, although these bacterial surface features influence the frequency of adhesion events and the early-stage detachment probability, none of them is responsible for the trend in the shear-enhanced adhesion time. These observations bring what we believe are new insights into the mechanism of bacterial attachment in shear flows, and suggest a role for other intrinsic features of the cell surface, or a dynamic cell response to shear stress.