Phylogeny and polymorphism in the E6 and E7 of human papillomavirus: alpha-9 (HPV16, 31, 33, 52, 58), alpha-5 (HPV51), alpha-6 (HPV53, 66), alpha-7 (HPV18, 39, 59, 68) and alpha-10 (HPV6, 44) in women from Shanghai
Phylogeny and polymorphism in the E6 and E7 of human papillomavirus: alpha-9 (HPV16, 31, 33, 52, 58), alpha-5 (HPV51), alpha-6 (HPV53, 66), alpha-7 (HPV18, 39, 59, 68) and alpha-10 (HPV6, 44) in women from Shanghai
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人乳头瘤病毒 E6 和 E7 的系统发育和多态性：α-9 (HPV16, 31, 33, 52, 58)，α-5 (HPV51)，α-6 (HPV53, 66)，α-7 (HPV18, 39)
DOI：
10.1186/s13027-019-0250-9
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发表时间：
2019-11-21
影响因子：
3.7
通讯作者：
Li, Fang
中科院分区：
文献类型：
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作者：
Zhao, Junwei;Zhan, Qin;Li, Fang
背景：人乳头瘤病毒（HPV）持续感染与宫颈上皮内瘤变（CIN）和宫颈癌有关。然而，为什么只有一小部分HPV病例进展为癌症仍不清楚。方法对262例HPV阳性宫颈病变患者中14种常见HPV型别的异质性、分类、演变及变异分布进行分析。对HPV E6、E7基因进行序列测定，并与HPV参考株进行比较分析。使用MEGA 7.0的邻接树方法构建系统发育树。结果本研究共扩增出233条E6和212条E7序列，并将其分为5个种群：α 9（HPV 16、31、33、52、58）、α 5（HPV 51）、α 6（HPV 53、66）、α 7（HPV 18、39、59、68）和α 10（HPV 6、44）。α 9型HPV感染者的高度鳞状上皮内病变（HSIL）发生率明显高于其他各组（P < 0.0001），尤其是HPV16型（P < 0.0001）。令人惊讶的是，E7在HSIL中的非同义变异明显少于
Background Persistent infection with human papillomaviruses (HPVs) has been associated with cervical intraepithelial neoplasia (CIN) and cervical cancer. However, why only a fraction of HPV cases progress to cancer is still unclear. Methods We focused on the heterogeneity, classification, evolution and dispersal of variants for 14 common HPV types in 262 HPV-positive patients with cervical lesions. The E6 and E7 genes of HPV were sequenced and compared with the HPV reference for sequence analysis. Phylogenetic trees were constructed using the neighbour-joining tree method with MEGA 7.0. Results In this study, 233 E6 and 212 E7 sequences were successfully amplified by PCR, and these sequences were divided into 5 species groups: alpha-9 (HPV16, 31, 33, 52, 58), alpha-5 (HPV51), alpha-6 (HPV53, 66), alpha-7 (HPV18, 39, 59, 68) and alpha-10 (HPV6, 44). The incidence of high-grade squamous intraepithelial lesion (HSIL) in patients infected with alpha-9 HPV was significantly increased compared with other groups (P < 0.0001), especially HPV16 (P < 0.0001). Strikingly, E7 had significantly fewer nonsynonymous variants in the HSIL compared to