Structural features of a polygalacturonase gene cloned from Aspergillus oryzae KBN616.
Structural features of a polygalacturonase gene cloned from Aspergillus oryzae KBN616.
复制标题
从米曲霉 KBN616 克隆的多聚半乳糖醛酸酶基因的结构特征。
DOI：
10.1111/j.1574-6968.1993.tb06358.x
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发表时间：
1993
影响因子：
2.1
通讯作者：
N. Tsukagoshi
中科院分区：
文献类型：
--
作者：
N. Kitamoto;T. Kimura;Y. Kito;K. Ohmiya;N. Tsukagoshi
对酱料生产中使用的曲霉多聚半乳糖醛酸酶基因组进行了克隆和序列测定。该结构基因全长1227 bp，编码363个氨基酸，含有28个氨基酸的前肽，开放阅读框被57 bp和81 bp的短内含子所破坏。推导的成熟蛋白氨基酸序列与尼日尔曲霉多聚半乳糖醛酸酶Ⅰ、黑曲霉多聚半乳糖醛酸酶Ⅱ、塔宾曲霉多聚半乳糖醛酸酶Ⅱ和炭旋孢菌多聚半乳糖醛酸酶的氨基酸序列同源性分别为63%、63%、63%和64%。然而，真菌、植物和细菌多聚半乳糖醛酸酶之间几乎没有同源性。
A genomic gene encoding a polygalacturonase from Aspergillus oryzae, used in soy sauce production, was cloned and sequenced. The structural gene comprises 1227 bp coding for 363 amino acids with a putative prepropeptide of 28 amino acids and the open reading frame is disrupted by two short introns of 57 bp and 81 bp. The deduced amino acid sequence of the mature protein showed 63, 63, 63 and 64% homology with those of Aspergillus niger polygalacturonase I, Aspergillus niger polygalacturonase II, Aspergillus tubingensis polygalacturonase II and Cochliobolus carbonum polygalacturonase, respectively. There is, however, little homology among fungal, plant and bacterial polygalacturonases.