Trephor: A new tool for sampling microcores from tree stems
Trephor: A new tool for sampling microcores from tree stems
复制标题
DOI：
10.1163/22941932-90000139
复制
发表时间：
2006-01-01
期刊：
影响因子：
1.9
通讯作者：
Menardi, R
中科院分区：
文献类型：
--
作者：
Rossi, S;Anfodillo, T;Menardi, R
对生长过程中形成层活动和木材形成的详细分析需要对新形成的木质部进行重复采样。为了尽可能减少侵入性，木材样品被提取为微核。尽管对木质部细胞发育进行了研究，并且对年度内木质发生研究的兴趣日益浓厚，但可用于微核心取样的工具却很少。最初为其他目的而设计的方法经常被使用，但没有关于其效率和有用性的详细信息。还缺乏用于树木年轮形成细胞分析的实验室制备技术的信息，导致进行这些实验的困难。描述了迄今为止使用的工具的优点和局限性。推出了一种名为 Trephor（正在申请专利 n 度 PD2004A000324）的新工具，专为长期使用而设计。 Trephor 呈凿子形状，可快速恢复 2 毫米直径的微芯。使用锤子将切割管插入木材中，不需要其他配件。简单的技术特征允许从软木和硬木树种中采集高质量的样品，同时对采样树木的损害最小。 Trephor 在 2004 年生长季节进行了测试，显示出良好的耐磨性和机械应力性。描述了将微核包埋在石蜡中以进行快速切片制备。
Detailed analyses of cambium activity and wood formation during growth need repeated sampling of newly formed xylem. In order to be minimally invasive, wood samples are extracted as rnicrocores. Despite the research done on xylem cell development and the increasing interest in intra-annual studies of xylogenesis, few tools are available for microcore sampling. Methods originally designed for other purposes have often been used, but no details are available on their efficiency and usefulness. Information is also lacking on laboratory preparation techniques for cell analysis of tree-ring formation, leading to difficulties in carrying out these experiments. The advantages and limits of the tools used up to now are described. A new tool, named Trephor (patent pending n degrees PD2004A000324), specifically designed for long-lasting use is presented. Trephor is chisel-shaped for a fast recovery of 2 mm diameter microcores. The cutting tube is inserted into the wood using a hammer and no other accessory is required. Simple technical characteristics allow high quality samples to be collected from both softwood and hardwood species with minimum damage to the sampled trees. Trephor was tested during the 2004 growing season, demonstrating good resistance to wear and tear and mechanical stress. Embedding the microcores in paraffin for fast section preparation is described.